Genetic transformation of the green alga -: Chlamydomonas reinhardtii by Agrobacterium tumefaciens

被引：169

作者：

Kumar, SV

Misquitta, RW

Reddy, VS

Rao, BJ

Rajam, MV

机构：

[1] Univ Delhi, Dept Genet, Plant Polyamine & Transgen Res Lab, New Delhi 110021, India

[2] Tata Inst Fundamental Res, Dept Sci Biol, Bombay 400005, Maharashtra, India

[3] Int Ctr Genet Engn & Biotechnol, New Delhi 110067, India

来源：

PLANT SCIENCE | 2004年 / 166卷 / 03期

关键词：

Chlamydomonas; genetic transformation; Agrobacterium;

D O I：

10.1016/j.plantsci.2003.11.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the first successful transfer of T-DNA of Agrobacterium tumefaciens carrying the genes coding for P-glucuronidase (uidA), green fluorescent protein (gfp) and hygromycin phosphotransferase (hpt) to the nuclear genome of the green alga Chlamydomonas reinhardtii. The transformation frequency (which was based on hygromycin resistance phenotype) was 50-fold higher than that of the glass bead transformation. Molecular and genetic analyses performed on transformants revealed the stable nuclear integration and expression of transgenes. The simplicity of Agrobacterium-mediated gene transfer and the high transformation frequency as well as the precision of T-DNA integration will enable further molecular dissection of this important model organism to understand basic plant metabolic processes as well as to exploit the system for biotechnological applications. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：731 / 738

页数：8

共 22 条

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