Characterisation of human peroxisomal 2,4-dienoyl-CoA reductase

被引：11

作者：

De Nys, K ^{[1
]}

Meyhi, E ^{[1
]}

Mannaerts, GP ^{[1
]}

Fransen, M ^{[1
]}

Van Veldhoven, PP ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Afdeling Farmacol, B-3000 Louvain, Belgium

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2001年 / 1533卷 / 01期

关键词：

beta-oxidation; docosahexaenoic acid; peroxisome; PTS1; polyunsaturated fatty acid;

D O I：

10.1016/S1388-1981(01)00141-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the primary structure of the rat peroxisomal 2,4-dienoyl-CoA reductase (M. Fransen, P.P. Van Veldhoven, S. Subramani, Biochem. J. 340 (1999) 561-568), the cDNA of the human counterpart was cloned. It contained an open reading frame of 878 bases encoding a protein of 291 amino acids (calculated molecular mass 30 778 Da), being 83% identical to the rat reductase. The gene, encompassing nine exons, is located at chromosome 16p13. Bacterially expressed poly(His)tagged reductase was active not only towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA. Hence, the reductase does not seem to constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. The reduction of docosaheptaenoyl-CoA, however, was severely decreased in the presence of albumin. (C) 2001 Elsevier Science B.V. Al rights reserved.

引用

页码：66 / 72

页数：7

共 25 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

AMERY L, 2001, IN PRESS COMB CHEM H

[3] ESTABLISHING A HUMAN TRANSCRIPT MAP [J].

BOGUSKI, MS ;

SCHULER, GD .

NATURE GENETICS, 1995, 10 (04) :369-371

[4] MITOCHONDRIAL AND PEROXISOMAL OXIDATION OF ARACHIDONIC AND EICOSAPENTAENOIC ACID STUDIED IN ISOLATED LIVER-CELLS [J].

CHRISTENSEN, E ;

HAGVE, TA ;

CHRISTOPHERSEN, BO .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 879 (03) :313-321

[5] Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs [J].

Das, AK ;

Uhler, MD ;

Hajra, AK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (32) :24333-24340

[6]

DOMMES V, 1981, J BIOL CHEM, V256, P8259

[7] Δ3,5-Δ2,4-dienoyl-CoA isomerase from rat liver -: Molecular characterization [J].

Filppula, SA ;

Yagi, AI ;

Kilpeläinen, SH ;

Novikov, D ;

FitzPatrick, DR ;

Vihinen, M ;

Valle, D ;

Hiltunen, JK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (01) :349-355

[8] Visualization of peroxisomal proteins containing a C-terminal targeting sequence on western blot by using the biotinylated PTS1-receptor [J].

Fransen, M ;

Brees, C ;

VanVeldhoven, PP ;

Mannaerts, GP .

ANALYTICAL BIOCHEMISTRY, 1996, 242 (01) :26-30

[9] Identification of peroxisomal proteins by using M13 phage protein VI phage display: molecular evidence that mammalian peroxisomes contain a 2,4-dienoyl-CoA reductase [J].

Fransen, M ;

Van Veldhoven, PP ;

Subramani, S .

BIOCHEMICAL JOURNAL, 1999, 340 :561-568

[10] The mouse gene PDCR encodes a peroxisomal Δ2,Δ4-dienoyl-CoA reductase [J].

Geisbrecht, BV ;

Liang, XQ ;

Morrell, JC ;

Schulz, H ;

Gould, SJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (36) :25814-25820

← 1 2 3 →