Violet pigment production from liquid pineapple waste by Chromobacterium violaceum UTM5 and evaluation of its bioactivity

被引:50
作者
Aruldass, Claira Arul [1 ]
Rubiyatno [1 ]
Venil, Chidambaram Kulandaisamy [1 ]
Ahmad, Wan Azlina [1 ]
机构
[1] Univ Teknol Malaysia, Fac Sci, Dept Chem, Utm Johor Bahru 81310, Johor, Malaysia
来源
RSC ADVANCES | 2015年 / 5卷 / 64期
关键词
LACTIC-ACID PRODUCTION; BACTERIAL PIGMENTS; BATCH FERMENTATION; LEAF EXTRACTS; GENE-CLUSTER; BIOSYNTHESIS; ANTIBACTERIAL; ANTIOXIDANT; EXPRESSION; TRYPTOPHAN;
D O I
10.1039/c5ra05765e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Synthetic pigments have been utilized in numerous industries including textile, cosmetic, food and pharmaceuticals. However, the drawbacks of these pigments, namely toxicity problems have kindled interest in natural pigments. In view of this, the use of natural pigments such as those from a bacterial origin offers an interesting alternative for industrial application. However, large scale applications of natural pigments are often hindered by the high production cost. This study evaluates the feasibility of using liquid pineapple waste for the production of violacein by a locally isolated Chromobacterium violaceum UTM5 both in a shake flask and a 50 L bioreactor. The use of optimized growth parameters including culture conditions, concentration of liquid pineapple waste and supplementation of L-tryptophan resulted in a violacein yield of 16 256 +/- 440 mg L-1. Post treatment of the effluent effectively reduced the COD, turbidity and TSS contents to less than 1 mg L-1, 1.57 +/- 0.2 NTU and 2.7 +/- 0.6 mg L-1 respectively. The violet pigment exhibited good stability during the entire storage period of 30 days at pH 7, temperature 25-30 degrees C and under dark conditions. The violet pigment has a good antimicrobial activity against selected microorganisms. Of interest, the pigment was active against Staphylococcus aureus ATCC 29213 and methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300 with a MIC value of 7.8 and 15.6 mu g mL(-1), respectively. However, the pigment is toxic to the V79-4 Chinese hamster lung cells with low selectivity index. The purified compounds were determined as violacein and deoxyviolacein respectively using FT-IR, LC-MS and NMR. The results confirmed the feasibility of using liquid pineapple waste as a potential low cost growth medium for the large-scale cultivation of violet pigment using C. violaceum UTM5.
引用
收藏
页码:51524 / 51536
页数:13
相关论文
共 50 条
[1]   Improved lactic acid productivity by an open repeated batch fermentation system using Enterococcus mundtii QU 25 [J].
Abdel-Rahman, Mohamed Ali ;
Tashiro, Yukihiro ;
Zendo, Takeshi ;
Sonomoto, Kenji .
RSC ADVANCES, 2013, 3 (22) :8437-8445
[2]   Production and Characterization of Violacein by Locally Isolated Chromobacterium violaceum Grown in Agricultural Wastes [J].
Ahmad, Wan Azlina ;
Yusof, Nur Zulaikha ;
Nordin, Nordiana ;
Zakaria, Zainul Akmar ;
Rezali, Mohd Fazlin .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 167 (05) :1220-1234
[3]   Pilot-scale removal of chromium from industrial wastewater using the ChromeBac™ system [J].
Ahmad, Wan Azlina ;
Zakaria, Zainul Akmar ;
Khasim, Ali Reza ;
Alias, Muhamad Anuar ;
Ismail, Shaik Muhammad Hasbullah Shaik .
BIORESOURCE TECHNOLOGY, 2010, 101 (12) :4371-4378
[4]  
[Anonymous], 2012, LPNM ANN REPORT
[5]   Brown sugar as a low-cost medium for the production of prodigiosin by locally isolated Serratia marcescens UTM1 [J].
Aruldass, Claira Arul ;
Venil, Chidambaran Kulandaisamy ;
Zakaria, Zainul Akmar ;
Ahmad, Wan Azlina .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2014, 95 :19-24
[6]   Effects of Mesua ferrea Leaf and Fruit Extracts on Growth and Morphology of Staphylococcus aureus [J].
Aruldass, Claira Arul ;
Marimuthu, Mani Maran ;
Ramanathan, Surash ;
Mansor, Sharif Mahsufi ;
Murugaiyah, Vikneswaran .
MICROSCOPY AND MICROANALYSIS, 2013, 19 (01) :254-260
[7]   In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum [J].
Balibar, Carl J. ;
Walsh, Christopher T. .
BIOCHEMISTRY, 2006, 45 (51) :15444-15457
[8]   Optimization of fermentation conditions for green pigment production from Bacillus cereus M161 (MTCC 5521) and its pharmacological application [J].
Banerjee, D. ;
Mondal, A. ;
Gupta, M. ;
Guha, A. K. ;
Ray, L. .
LETTERS IN APPLIED MICROBIOLOGY, 2014, 58 (01) :25-30
[9]   Cloning and heterologous expression of a natural product biosynthetic gene cluster from eDNA [J].
Brady, SF ;
Chao, CJ ;
Handelsman, J ;
Clardy, J .
ORGANIC LETTERS, 2001, 3 (13) :1981-1984
[10]   Magenta pigment produced by fungus [J].
Chiba, Sanae ;
Tsuyoshi, Naoko ;
Fudou, Ryosuke ;
Ojika, Makoto ;
Murakami, Yoshimasa ;
Ogoma, Yoshiro ;
Oguchi, Masakatu ;
Yamanaka, Shigeru .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2006, 52 (04) :201-207