Fine mapping of the Co-4 locus of common bean reveals a resistance gene candidate, COK-4, that encodes for a protein kinase

被引:41
作者
Melotto, M [1 ]
Kelly, JD [1 ]
机构
[1] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA
关键词
molecular markers; serine threonine kinase; single nucleotide polymorphism; homologous sequences; disease resistance gene;
D O I
10.1007/s001220100609
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The SAS13 SCAR marker, tightly linked with the CO-4(2) gene segregating in a population of 1018 F-2 individual plants, was used as a starting point for cloning gene sequences associated with the Co-4 locus that conditions resistance to anthracnose caused by the fungal pathogen Colletotrichum lindemuthianum in common bean (Phaseolus vulgaris). A contig developed from genomic clones flanking the marker region revealed a 1110-bp open reading frame, named COK-4. The predicted COK-4 protein contains a serine-threonine kinase domain highly similar to the protein encoded by the Pto gene in tomato, but with a highly hydrophobic membrane-spanning region. COK-4 homologs were cloned and sequenced from different bean cultivars. Single nucleotide polymorphisms were found between the homologous sequences and were confirmed with three restriction enzymes. Restriction patterns among three bean cultivars known to possess different alleles at the Co-4 locus, SEL 1308 (Co-4(2)), TO (Co-4) and Black Magic (co-4), were polymorphic. Absolute co-segregation between COK-4 restriction patterns and the disease phenotype was observed in 96 F-3 families. More than one copy of the COK-4 gene homolog exists in the bean genome as demonstrated by Southern analysis. These results suggest that COK-4 is part of the Co-4 locus conditioning resistance to C. lindemuthianum in bean.
引用
收藏
页码:508 / 517
页数:10
相关论文
共 44 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [3] Inheritance of anthracnose resistance in common bean differential cultivar AB 136
    AlzateMarin, AL
    Baia, GS
    dePaula, TJ
    deCarvalho, GA
    deBarros, EG
    Moreira, MA
    [J]. PLANT DISEASE, 1997, 81 (09) : 996 - 998
  • [4] Microsatellites and a single-nucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germ plasm
    Ayres, NM
    McClung, AM
    Larkin, PD
    Bligh, HFJ
    Jones, CA
    Park, WD
    [J]. THEORETICAL AND APPLIED GENETICS, 1997, 94 (6-7) : 773 - 781
  • [5] Bailey J. A., 1992, Colletotrichum: biology, pathology and control., P88
  • [6] Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central, and North America
    Balardin, RS
    Jarosz, AM
    Kelly, JD
    [J]. PHYTOPATHOLOGY, 1997, 87 (12) : 1184 - 1191
  • [7] Interaction between Colletotrichum lindemuthianum races and gene pool diversity in Phaseolus vulgaris
    Balardin, RS
    Kelly, JD
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1998, 123 (06) : 1038 - 1047
  • [8] Bent AF, 1996, PLANT CELL, V8, P1757, DOI 10.1105/tpc.8.10.1757
  • [9] Creusot F, 1999, GENOME, V42, P254, DOI 10.1139/gen-42-2-254
  • [10] The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins
    Dixon, MS
    Jones, DA
    Keddie, JS
    Thomas, CM
    Harrison, K
    Jones, JDG
    [J]. CELL, 1996, 84 (03) : 451 - 459