Quantitative and Qualitative RNA-Seq-Based Evaluation of Epstein-Barr Virus Transcription in Type I Latency Burkitt's Lymphoma Cells

被引：38

作者：

Lin, Zhen ^{[1
,2
]}

Xu, Guorong ^{[3
]}

Deng, Nan ^{[3
]}

Taylor, Christopher ^{[3
,4
]}

Zhu, Dongxiao ^{[3
,4
]}

Flemington, Erik K. ^{[1
,2
]}

机构：

[1] Tulane Univ, Hlth Sci Ctr, Dept Pathol, New Orleans, LA 70112 USA

[2] Tulane Canc Ctr, New Orleans, LA 70112 USA

[3] Univ New Orleans, Dept Comp Sci, New Orleans, LA 70148 USA

[4] Childrens Hosp, Res Inst Children, New Orleans, LA 70118 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 24期

关键词：

GENE-EXPRESSION; REPLICATION; INFECTION;

D O I：

10.1128/JVI.01521-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNA-seq provides a rich source of transcriptome information with high qualitative and quantitative value. Here, we provide a pipeline for Epstein-Barr virus (EBV) transcriptome analysis using RNA-seq and we apply it to two type I latency cell lines, Mutu I and Akata. This analysis revealed substantial average expression levels of many lytic genes in predominantly latent cell populations. The lytic transcripts BHLF1 and LF3 were expressed at levels greater than those for 98% of all cellular polyadenylated transcripts. Exon junction mapping accurately identified the Qp-derived EBNA1 splicing pattern, lytic gene splicing, and a complex splicing pattern within the BamHI A region.

引用

页码：13053 / 13058

页数：6

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