Cloning, sequencing and linkage mapping of the NRAMP1 gene of sheep and deer

被引:29
作者
Matthews, GD [1 ]
Crawford, AM [1 ]
机构
[1] Univ Otago, Dept Biochem, Agres Mol Biol Unit, Dunedin, New Zealand
关键词
deer; intracellular bacteria; microsatellite; NRAMP1; sheep;
D O I
10.1046/j.1365-2052.1998.00224.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The ovine NRAMP1 and cervine NRAMP1 cDNAs were cloned by RT PCR of RNA derived from macrophage enriched leukocyte preparations. The complete coding and 3' regions were sequenced. Both sheep and deer NRAMP1 proteins are 548 amino acids long. There are 77 and 73 amino acid differences, respectively, compared to the mouse Nramp1 sequence. Dinucleotide repeats were found in both the ovine and cervine 3' non-coding sequence. Amplification of these regions in individual sheep and deer showed them to be polymorphic microsatellites. They have polymorphism information content values of 0.76 and 0.84 in sheep and deer, respectively. Using these microsatellites, the ovine NRAMP1 gene was mapped in a linkage group on ovine chromosome 2q and cervine NRAMP1 was mapped in a linkage group syntenic with human chromosome 2, mouse chromosome 1 and sheep chromosome 2.
引用
收藏
页码:1 / 6
页数:6
相关论文
共 31 条
[1]   NH2-TERMINAL SEQUENCE OF MACROPHAGE-EXPRESSED NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN (NRAMP) ENCODES A PROLINE/SERINE-RICH PUTATIVE SRC HOMOLOGY 3-BINDING DOMAIN [J].
BARTON, CH ;
WHITE, JK ;
ROACH, TIA ;
BLACKWELL, JM .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (05) :1683-1687
[2]   NRAMP TRANSFECTION TRANSFERS ITY/LSH/BCG-RELATED PLEIOTROPIC EFFECTS ON MACROPHAGE ACTIVATION - INFLUENCE ON OXIDATIVE BURST AND NITRIC-OXIDE PATHWAYS [J].
BARTON, CH ;
WHITEHEAD, SH ;
BLACKWELL, JM .
MOLECULAR MEDICINE, 1995, 1 (03) :267-279
[3]   Structure and function of the natural-resistance-associated macrophage protein (Nramp1), a candidate protein for infectious and autoimmune disease susceptibility [J].
Blackwell, JM .
MOLECULAR MEDICINE TODAY, 1996, 2 (05) :205-211
[4]  
BOTSTEIN D, 1980, AM J HUM GENET, V32, P314
[5]  
CARTER CE, 1993, P DEER COURS VET, P208
[6]   HUMAN NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN - CDNA CLONING, CHROMOSOMAL MAPPING, GENOMIC ORGANIZATION, AND TISSUE-SPECIFIC EXPRESSION [J].
CELLIER, M ;
GOVONI, G ;
VIDAL, S ;
KWAN, T ;
GROULX, N ;
LIU, J ;
SANCHEZ, F ;
SKAMENE, E ;
SCHURR, E ;
GROS, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (05) :1741-1752
[7]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[8]  
CRAWFORD AM, 1995, GENETICS, V140, P703
[9]   Bovine natural resistance associated macrophage protein 1 (Nramp1) gene [J].
Feng, JW ;
Li, YJ ;
Hashad, M ;
Schurr, E ;
Gros, P ;
Adams, LG ;
Templeton, JW .
GENOME RESEARCH, 1996, 6 (10) :956-964
[10]   RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].
FROHMAN, MA ;
DUSH, MK ;
MARTIN, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002