Serum response factor-GATA ternary complex required for nuclear signaling by a G-protein-coupled receptor

被引:88
作者
Morin, S
Paradis, P
Aries, A
Nemer, M
机构
[1] Inst Rech Clin Montreal, Lab Dev & Differenciat Cardia, Montreal, PQ H2W 1R7, Canada
[2] Univ Montreal, Program Mol Biol, Montreal, PQ, Canada
[3] Univ Montreal, Dept Pharmacol, Montreal, PQ H3C 3J7, Canada
基金
中国国家自然科学基金;
关键词
D O I
10.1128/MCB.21.4.1036-1044.2001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Endothelins are a family of biologically active peptides that are critical for development and function of neural crest-derived and cardiovascular cells. These effects are mediated by two G-protein-coupled receptors and involve transcriptional regulation of growth-responsive and/or tissue-specific genes. We have used the cardiac ANF promoter, which represents the best-studied tissue-specific endothelin target, to elucidate the nuclear pathways responsible for the transcriptional effects of endothelins. We found that cardiac-specific response to endothelin 1 (ET-1) requires the combined action of the serum response factor (SRF) and the tissue-restricted GATA proteins which bind over their adjacent sites, within a 30-bp ET-1 response element. We show that SRF and GATA proteins form a novel ternary complex reminiscent of the well-characterized SRF-ternary complex factor interaction required for transcriptional induction of c-fos in response to growth factors. In transient cotransfections, GATA factors and SRF synergistically activate atrial natriuretic factor and other ET-1-inducible promoters that contain both GATA and SRF binding sites. Thus, GATA factors may represent a new class of tissue-specific SRF accessory factors that account for muscle- and other cell-specific SRF actions.
引用
收藏
页码:1036 / 1044
页数:9
相关论文
共 64 条
[1]   CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].
ARAI, H ;
HORI, S ;
ARAMORI, I ;
OHKUBO, H ;
NAKANISHI, S .
NATURE, 1990, 348 (6303) :730-732
[2]   A NUCLEAR PATHWAY FOR ALPHA(1)-ADRENERGIC RECEPTOR SIGNALING IN CARDIAC-CELLS [J].
ARDATI, A ;
NEMER, M .
EMBO JOURNAL, 1993, 12 (13) :5131-5139
[3]  
ARGENTIN S, 1991, J BIOL CHEM, V266, P23315
[4]   DEVELOPMENTAL STAGE-SPECIFIC REGULATION OF ATRIAL-NATRIURETIC-FACTOR GENE-TRANSCRIPTION IN CARDIAC-CELLS [J].
ARGENTIN, S ;
ARDATI, A ;
TREMBLAY, S ;
LIHRMANN, I ;
ROBITAILLE, L ;
DROUIN, J ;
NEMER, M .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (01) :777-790
[5]   GROWTH REGULATORY PROPERTIES OF ENDOTHELINS [J].
BATTISTINI, B ;
CHAILLER, P ;
DORLEANSJUSTE, P ;
BRIERE, N ;
SIROIS, P .
PEPTIDES, 1993, 14 (02) :385-399
[6]   INTERACTION OF ENDOTHELIN-3 WITH ENDOTHELIN-B RECEPTOR IS ESSENTIAL FOR DEVELOPMENT OF EPIDERMAL MELANOCYTES AND ENTERIC NEURONS [J].
BAYNASH, AG ;
HOSODA, K ;
GIAID, A ;
RICHARDSON, JA ;
EMOTO, N ;
HAMMER, RE ;
YANAGISAWA, M .
CELL, 1994, 79 (07) :1277-1285
[7]  
BOGOYEVITCH MA, 1994, J BIOL CHEM, V269, P1110
[8]   THE SARCOMERIC ACTIN CARG-BINDING FACTOR IS INDISTINGUISHABLE FROM THE C-FOS SERUM RESPONSE FACTOR [J].
BOXER, LM ;
PRYWES, R ;
ROEDER, RG ;
KEDES, L .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (02) :515-522
[9]   The developmentally regulated expression of serum response factor plays a key role in the control of smooth muscle-specific genes [J].
Browning, CL ;
Culberson, DE ;
Aragon, IV ;
Fillmore, RA ;
Croissant, JD ;
Schwartz, RJ ;
Zimmer, WE .
DEVELOPMENTAL BIOLOGY, 1998, 194 (01) :18-37
[10]   DISTINCT BEHAVIOR OF CARDIAC MYOSIN HEAVY-CHAIN GENE CONSTRUCTS INVIVO - DISCORDANCE WITH INVITRO RESULTS [J].
BUTTRICK, PM ;
KAPLAN, ML ;
KITSIS, RN ;
LEINWAND, LA .
CIRCULATION RESEARCH, 1993, 72 (06) :1211-1217