Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

被引：282

作者：

Sekine, T

Cha, SH

Tsuda, M

Apiwattanakul, N

Nakajima, N

Kanai, Y

Endou, H ^{[1
]}

机构：

[1] Kyorin Univ, Sch Med, Dept Pharmacol & Toxicol, Mitaka, Tokyo 181, Japan

[2] Catholic Univ, Coll Med, Dept Pharmacol, Seocho Gu, Seoul 137701, South Korea

来源：

FEBS LETTERS | 1998年 / 429卷 / 02期

关键词：

liver; multispecific transporter; organic anion; pharmacokinetics;

D O I：

10.1016/S0014-5793(98)00585-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1, When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE(2), dicarboxylates and p-aminohippurate, [C-14]Salicylate uptake via NLT was saturable (K-m = 88.8 +/- 23.4 mu M) and sodium-independent, Expression of the mRNA of NLT was detected in the liver and kidney (liver much greater than kidney). We propose that NLT be renamed OAT2. (C) 1998 Federation of European Biochemical Societies.

引用

页码：179 / 182

页数：4

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