Serotyping of Cryptococcus neoformans by dot enzyme assay

被引：24

作者：

Belay, T

Cherniak, R

ONeill, EB

Kozel, TR

机构：

[1] GEORGIA STATE UNIV,DEPT CHEM,LAB BIOL & CHEM SCI,ATLANTA,GA 30303

[2] UNIV NEVADA,DEPT MICROBIOL,RENO,NV 89557

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 02期

关键词：

D O I：

10.1128/JCM.34.2.466-470.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.

引用

收藏

页码：466 / 470

页数：5

相关论文

共 30 条

[1]

STRUCTURE OF THE O-DEACETYLATED GLUCURONOXYLOMANNAN FROM CRYPTOCOCCUS-NEOFORMANS SEROTYPE-C AS DETERMINED BY 2D H-1-NMR SPECTROSCOPY [J].

BACON, BE ;

CHERNIAK, R .

CARBOHYDRATE RESEARCH, 1995, 276 (02) :365-386

[2]

SPECIFICITY OF CRYPTOCOCCUS-NEOFORMANS FACTOR SERA DETERMINED BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY AND DOT ENZYME ASSAY [J].

BELAY, T ;

CHERNIAK, R ;

SHINODA, T .

INFECTION AND IMMUNITY, 1993, 61 (07) :2879-2885

[3]

BENNETT JE, 1965, J IMMUNOL, V94, P916

[4]

BHATTACHARJEE AK, 1984, REV INFECT DIS, V6, P619

[5]

SEROGROUP DISTRIBUTION OF CRYPTOCOCCUS-NEOFORMANS IN PATIENTS WITH AIDS [J].

BOTTONE, EJ ;

SALKIN, IF ;

HURD, NJ ;

WORMSER, GP .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (01) :242-242

[6]

MULTILOCUS ENZYME TYPING OF CRYPTOCOCCUS-NEOFORMANS [J].

BRANDT, ME ;

BRAGG, SL ;

PINNER, RW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) :2819-2823

[7]

EXTENSIVE ALLELIC VARIATION IN CRYPTOCOCCUS-NEOFORMANS [J].

CASADEVALL, A ;

FREUNDLICH, LF ;

MARSH, L ;

SCHARFF, MD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (05) :1080-1084

[8]

FACILITATED ISOLATION, PURIFICATION, AND ANALYSIS OF GLUCURONOXYLOMANNAN OF CRYPTOCOCCUS-NEOFORMANS [J].

CHERNIAK, R ;

MORRIS, LC ;

ANDERSON, BC ;

MEYER, SA .

INFECTION AND IMMUNITY, 1991, 59 (01) :59-64

[9]

VARIATION IN THE STRUCTURE OF GLUCURONOXYLOMANNAN IN ISOLATES FROM PATIENTS WITH RECURRENT CRYPTOCOCCAL MENINGITIS [J].

CHERNIAK, R ;

MORRIS, LC ;

BELAY, T ;

SPITZER, ED ;

CASADEVALL, A .

INFECTION AND IMMUNITY, 1995, 63 (05) :1899-1905

[10]

GLUCURONOXYLOMANNAN OF CRYPTOCOCCUS-NEOFORMANS SEROTYPE-C - STRUCTURAL-ANALYSIS BY GAS-LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND C-13-NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY [J].

CHERNIAK, R ;

MORRIS, LC ;

MEYER, SA .

CARBOHYDRATE RESEARCH, 1992, 225 (02) :331-337