Ex vivo expansion of hematopoietic stem cells in bioreactors

被引：26

作者：

Cabral, JMS ^{[1
]}

机构：

[1] Univ Tecn Lisboa, Ctr Engn Biol & Quim, Inst Super Tecn, P-1049001 Lisbon, Portugal

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 10期

关键词：

differentiation; expansion; hollow fiber reactors; packed bed reactors; perfusion reactors; stem cells; stirred reactors;

D O I：

10.1023/A:1010350215989

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The in vitro culture of human hematopoietic stem cells in different types of bioreactors is reviewed. After a brief description of the hematopoiesis and stem cells biology, the selection of growth medium for stem cells is evaluated as well as the parameters to be controlled for ex vivo expansion and differentiation of stem cells. Several types of bioreactors for in vitro stem cell culture are discussed and compared.

引用

页码：741 / 751

页数：11

共 41 条

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