Sequencing and analysis of the Edwardsiella ictaluri plasmids

To determine possible functions of the Edwardsiella ictaluri plasmids, pEI1 and pEI2, we analyzed the sequence of both plasmids. Plasmid pEI1 is 4807 bp, with 51% G + C, and 23 possible open reading frames of 40 amino acids or greater Plasmid pEI2 is 5643 bp, with 51% G + C, and 24 possible reading frames. Database searches indicated that pEI1 contains an insertion clement and a ROM analog. In addition. pEI1 possesses an open reading frame with strong homology to SlrP, SspH1, and SspH2 of Salmonella typhimurium and IpaH of Shigella flexnari which have leucine-rich repeat regions and are components of type III secretory systems, pEI2 has a frame with weak homology to Spa15 of S. flexnari 5 and InvB of S. sonnei and S, typhimurium, which are also type III secretory system components, three origins of replication, a Rep analog, and a multimer resolution site. (C) 2001 Academic Press.