Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS

被引:92
作者
Widmer, G
Tzipori, S
Fichtenbaum, CJ
Griffiths, JK
机构
[1] Tufts Univ, Sch Vet Med, Div Infect Dis, North Grafton, MA 01536 USA
[2] Tufts Univ, Sch Med, Dept Family Med & Community Hlth, Boston, MA 02111 USA
[3] Tufts Univ New England Med Ctr, Div Geog Med, Boston, MA USA
[4] Washington Univ, Sch Med, Dept Internal Med, St Louis, MO 63110 USA
关键词
D O I
10.1086/515373
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Genotypic analysis of Cryptosporidiun parvum has demonstrated the presence of two subgroups within the species, whereas biochemical and antigenic characterization have shown more heterogeneity. The clinical relevance of these observations is unknown. C. parvum isolates from people with AIDS were studied with respect to parasite genotypes and virulence in cell monolayers and laboratory animals. Ten elf 13 oocyst samples had a characteristic human-associated (H) genotype; 3 had a genotype typic;il of calf-excreted oocysts (C), Virulence in cell culture was mildly or markedly lower in the 5 isolate:; tested (4 H and 1 C) compared with the GCH1 reference isolate, H isolates did not infect newborn ICR mice, whereas 1 of the 2 C isolates tested did. These findings reinforce the concept of C. parvum genetic subgroupings that correlate to some extent with infectivity and suggest that additional heterogeneity is present within the subgroups.
引用
收藏
页码:834 / 840
页数:7
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