Investigation on the temperature effect of a mixed vesicle composed of polydiacetylene and BODIPY 558

被引：9

作者：

Guo, Caixin ^{[1
]}

Zhang, Renzhe ^{[1
]}

Jiang, Long ^{[1
]}

Liu, Tao ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2007年 / 60卷 / 01期

基金：

中国国家自然科学基金;

关键词：

polydiacetylene; fluorescence; vesicle-based sensor;

D O I：

10.1016/j.colsurfb.2007.05.024

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

BODIPY 558 is an insensitive fluorescent reagent to temperature. But when it is inserted into polydiacetylene (PDA) vesicles, the resultant complex presents a considerable temperature effect. In this article, we reported the temperature-dependent fluorescence intensity of a vesicle-based sensor constructed by PDA and BODIPY fluorescent probe. The fluorescence of BODIPY was considerably quenched in the polymerized diacetylene lipid membrane, but recovered by increasing the temperature of vesicle solution. The mechanism of quenching was detailedly investigated, and we deduced that the fluorescence quenching and recovery were associated with the conjugated conformation of the PDA backbone. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：41 / 45

页数：5

共 13 条

[1] VISUAL CONFORMATIONAL TRANSITIONS OF WATER-SOLUBLE POLYDIACETYLENES - EFFECTS OF PH AND ELECTROLYTE ON ABSORPTION AND FLUORESCENCE-SPECTRA [J].