Real-time reporting of circadian-regulated gene expression by luciferase imaging in plants and mammalian cells

被引：68

作者：

Welsh, DK

Imaizumi, T

Kay, SA

机构：

[1] Department of Cell Biology, Scripps Research Institute, San Diego

[2] Department of Psychiatry, San Diego VA Medical Center, University of California, San Diego, San Diego

来源：

CIRCADIAN RHYTHMS | 2005年 / 393卷

关键词：

D O I：

10.1016/S0076-6879(05)93011-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Luciferase enzymes have been used as reporters of circadian rhythms in organisms as diverse as cyanobacteria, plants, fruit flies, and mice. This article details methodology for real-time reporting of circadian-regulated gene expression by imaging of luciferase bioluminescence in plants and mammalian cells.

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收藏

页码：269 / 288

页数：20

相关论文

共 30 条

[1]

[Anonymous], 2001, NEURON, DOI DOI 10.1016/S0896-6273(01)00302-6

[2] Visualization of mPer1 transcription in vitro:: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN [J].

Asai, M ;

Yamaguchi, S ;

Isejima, H ;

Jonouchi, M ;

Moriya, T ;

Shibata, S ;

Kobayashi, M ;

Okamura, H .

CURRENT BIOLOGY, 2001, 11 (19) :1524-1527

[3] Seeing the wood through the trees: A review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence [J].

Billinton, N ;

Knight, AW .

ANALYTICAL BIOCHEMISTRY, 2001, 291 (02) :175-197

[4] Novel features of Drosophila period transcription revealed by real-time luciferase reporting [J].

Brandes, C ;

Plautz, JD ;

Stanewsky, R ;

Jamison, CF ;

Straume, M ;

Wood, KV ;

Kay, SA ;

Hall, JC .

NEURON, 1996, 16 (04) :687-692

[5]

CHRISTENSON MA, 2002, LUMINESCENCE BIOTECH, P469

[6] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[7] Imaging of light emission from the expression of luciferases in living cells and organisms: a review [J].

Greer, LF ;

Szalay, AA .

LUMINESCENCE, 2002, 17 (01) :43-74

[8] Expression of Period genes:: Rhythmic and nonrhythmic compartments of the suprachiasmatic nucleus pacemaker [J].

Hamada, T ;

LeSauter, J ;

Venuti, JM ;

Silver, R .

JOURNAL OF NEUROSCIENCE, 2001, 21 (19) :7742-7750

[9] CHEMISTRY, CLONES, AND CIRCADIAN CONTROL OF THE DINOFLAGELLATE BIOLUMINESCENT SYSTEM [J].

HASTINGS, JW .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1989, 4 (01) :12-19

[10] Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons [J].

Ikeda, M ;

Sugiyama, T ;

Wallace, CS ;

Gompf, HS ;

Yoshioka, T ;

Miyawaki, A ;

Allen, CN .

NEURON, 2003, 38 (02) :253-263