Intracisternal TRH analog induces Fos expression in gastric myenteric neurons and glia in conscious rats

被引:35
作者
Miampamba, M
Yang, H
Sharkey, KA
Taché, Y
机构
[1] Univ Calif Los Angeles, Vet Affairs Greater Los Angeles Healthcare Syst, CURE Digest Dis Res Ctr, Los Angeles, CA 90073 USA
[2] Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90073 USA
[3] Univ Calif Los Angeles, Brain Res Inst, Los Angeles, CA 90073 USA
[4] Univ Calgary, Dept Physiol & Biophys, Neurosci Res Grp, Calgary, AB T2N 4N1, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2001年 / 280卷 / 05期
关键词
RX-77368; immediate-early gene; atropine; hexamethonium; vesicular acetylcholine transporter; enteric nervous system;
D O I
10.1152/ajpgi.2001.280.5.G979
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Activation of gastric myenteric cells by intracisternal injection of the stable thyrotropin-releasing hormone (TRH) analog RX-77368, at a dose inducing near maximal vagal cholinergic stimulation of gastric functions, was investigated in conscious rats. Fos immunoreactivity was assessed in gastric longitudinal muscle-myenteric plexus whole mount preparations 90 min after intracisternal injection. Fos-immunoreactive cells were rare in controls (similar to1 cell/ganglion), whereas intracisternal RX-77368 (50 ng) increased the number to 24.8 +/- 1.8 and 26.8 +/- 2.2 cells/ganglion in the corpus and antrum, respectively. Hexamethonium (20 mg/kg sc) prevented Fos expression by 90%, whereas atropine (2 mg/kg sc) had no effect. The neuronal marker protein gene product 9.5 and the glial markers S-100 and glial fibrillary acidic proteins showed that RX-77368 induced Fos in both myenteric neurons and glia. Vesicular ACh transporter and calretinin were detected around the activated myenteric neurons. These results indicated that central vagal efferent stimulation by intracisternal RX-77368 activates gastric myenteric neurons as well as glial cells mainly through nicotinic ACh receptors in conscious rats.
引用
收藏
页码:G979 / G991
页数:13
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