Characteristics of pullulanases from extremely thermophilic archaea isolated from deep-sea hydrothermal vents

被引：14

作者：

Gantelet, H

Ladrat, C

Godfroy, A

Barbier, G

Duchiron, F

机构：

[1] Univ Reims Champagne Ardenne, UFR Sci Exactes & Nat, Lab Microbiol Ind, F-51687 Reims 2, France

[2] IFREMER, Ctr Brest, Lab Biotechnol Microorganismes Hydrothermaux, DRP EP, F-29280 Plouzane, France

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 09期

关键词：

pullulanase; deep-sea hydrothermal vents; Thermococcus; thermostability; archaea;

D O I：

10.1023/A:1005399006318

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two out of three extremely thermophilic anaerobic archaea, isolated from deep-sea hydrothermal vents, produced pullulanase activity in the presence of maltose in the growth medium. Enzyme activities were mainly extracellular and characterized by optimum temperatures of 95 degrees C and 80-95 degrees C, optimum pH of 5.0-7.0 and a high degree of thermostability. One strain when grown in a fermenter with maltose as inducer produced pullulanase at 35 U/I.

引用

页码：819 / 823

页数：5

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