Multiplexed Parallel Single Transport Recordings on Nanopore Arrays

被引：60

作者：

Kleefen, Alexander ^{[1
,2
]}

Pedone, Daniel ^{[3
]}

Grunwald, Christian ^{[1
,2
]}

Wei, Ruoshan ^{[3
]}

Firnkes, Matthias ^{[3
]}

Abstreiter, Gerhard ^{[3
]}

Rant, Ulrich ^{[3
]}

Tampe, Robert ^{[1
,2
]}

机构：

[1] Goethe Univ Frankfurt, Ctr Membrane Proteom CMP, Bioctr, Inst Biochem, D-60438 Frankfurt, Germany

[2] Goethe Univ Frankfurt, Cluster Excellence Frankfurt, D-60438 Frankfurt, Germany

[3] Tech Univ Munich, Walter Schottky Inst, D-85748 Garching, Germany

来源：

NANO LETTERS | 2010年 / 10卷 / 12期

关键词：

Biosensor; membrane proteins; nanofabrication; lab-on-chip; single channel recording; SUPPORTED LIPID-BILAYERS; SOLID-STATE NANOPORES; PORE-FORMING PROTEIN; SILICON-NITRIDE; MEMBRANE; FABRICATION; CHANNEL; ELECTROPHYSIOLOGY; MICROARRAYS; KINETICS;

D O I：

10.1021/nl1033528

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of similar to 1000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.

引用

页码：5080 / 5087

页数：8

共 40 条

[1] The membrane-permeabilizing effect of avenacin A-1 involves the reorganization of bilayer cholesterol [J].

Armah, CN ;

Mackie, AR ;

Roy, C ;

Price, K ;

Osbourn, AE ;

Bowyer, P ;

Ladha, S .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :281-290

[2] Planar microelectrode-cavity array for high-resolution and parallel electrical recording of membrane ionic currents [J].

Baaken, Gerhard ;

Sondermann, Markus ;

Schlemmer, Christian ;

Ruehe, Juergen ;

Behrends, Jan C. .

LAB ON A CHIP, 2008, 8 (06) :938-944

[3] ALPHA-TOXIN OF STAPHYLOCOCCUS-AUREUS [J].

BHAKDI, S ;

TRANUMJENSEN, J .

MICROBIOLOGICAL REVIEWS, 1991, 55 (04) :733-751

[4] Silicon-on-insulator based nanopore cavity arrays for lipid membrane investigation [J].

Buchholz, K. ;

Tinazli, A. ;

Kleefen, A. ;

Dorfner, D. ;

Pedone, D. ;

Rant, U. ;

Tampe, R. ;

Abstreiter, G. ;

Tornow, M. .

NANOTECHNOLOGY, 2008, 19 (44)

[5] Solid supported lipid bilayers: From biophysical studies to sensor design [J].

Castellana, Edward T. ;

Cremer, Paul S. .

SURFACE SCIENCE REPORTS, 2006, 61 (10) :429-444

[6] A PHOTOGENERATED PORE-FORMING PROTEIN [J].

CHANG, CY ;

NIBLACK, B ;

WALKER, B ;

BAYLEY, H .

CHEMISTRY & BIOLOGY, 1995, 2 (06) :391-400

[7] Formation and spreading of lipid bilayers on planar glass supports [J].

Cremer, PS ;

Boxer, SG .

JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (13) :2554-2559

[8] Solid-state nanopores [J].

Dekker, Cees .

NATURE NANOTECHNOLOGY, 2007, 2 (04) :209-215

[9] High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology [J].

Dunlop, John ;

Bowlby, Mark ;

Peri, Ravikumar ;

Vasilyev, Dmytro ;

Arias, Robert .

NATURE REVIEWS DRUG DISCOVERY, 2008, 7 (04) :358-368

[10] Kinetics of escape through a small hole [J].

Grigoriev, IV ;

Makhnovskii, YA ;

Berezhkovskii, AM ;

Zitserman, VY .

JOURNAL OF CHEMICAL PHYSICS, 2002, 116 (22) :9574-9577

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