Molecular complex formation between riboflavin and salicylate in an aqueous medium

Evidence for an intermolecular interaction of riboflavin and sodium salicylate in an aqueous medium was obtained from the presence of isosbestic points in the absorption spectra and an isoemissive point in the emission spectra. The equilibrium constant was estimated from absorption and emission studies, and also from the solubility of riboflavin in an aqueous solution of sodium salicylate, and was found to be in the range of 10-14 mol dm(-3). Lifetime measurements indicate a predominantly static mechanism of quenching. The Stern-Volmer constant for the quenching of riboflavin by sodium salicylate was significantly reduced upon the addition Of Urea. An MD Simulation Study on the system revealed a stacked conformation, which is probably due to a hydrophobic interaction of the two components.