Isolation of an ent-kaurene oxidase cDNA from Cucurbita maxima

被引：20

作者：

Helliwell, CA

Olive, MR

Gebbie, L

Forster, R

Peacock, WJ

Dennis, ES

机构：

[1] CSIRO Plant Ind, Canberra, ACT 2601, Australia

[2] Australian Natl Univ, Div Biochem & Mol Biol, Canberra, ACT 0200, Australia

来源：

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY | 2000年 / 27卷 / 12期

关键词：

D O I：

10.1071/PP00079

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Four distinct cDNAs isolated from developing Cucurbita maxima Duchesne seeds included CYP701A1, a member of the same subfamily of cytochrome P450 enzyme as the Arabidopsis ent-kaurene oxidase GA3 which catalyses an early step in gibberellin (GA) biosynthesis. We have shown by complementation of the Arabidopsis ga3-2 mutant that the CYP701A1 cDNA encodes an ent-kaurene oxidase activity. Another cDNA, CYP88A2, encodes a protein which is in the same subfamily as the maize Dwarf3 protein and is likely to encode another GA biosynthetic enzyme catalysing a reaction between ent-kaurenoic acid and GA(12).

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页码：1141 / 1149

页数：9

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