Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P

被引：80

作者：

Plehn-Dujowich, D ^{[1
]}

Altman, S ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 13期

关键词：

D O I：

10.1073/pnas.95.13.7327

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. when synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.

引用

页码：7327 / 7332

页数：6

共 47 条

[1] RNA ENZYME-DIRECTED GENE-THERAPY [J].

ALTMAN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :10898-10900

[2]

BARCENA J, 1994, J VIROL, V68, P6900

[3] TRANSCRIPTION ANTITERMINATION DURING INFLUENZA VIRAL TEMPLATE RNA-SYNTHESIS REQUIRES THE NUCLEOCAPSID PROTEIN AND THE ABSENCE OF A 5' CAPPED END [J].

BEATON, AR ;

KRUG, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6282-6286

[4]

CARRAT F, 1994, REV MAL RESPIR, V11, P239

[5] UPSTREAM REGULATORY ELEMENTS ARE NECESSARY AND SUFFICIENT FOR TRANSCRIPTION OF A U6-RNA GENE BY RNA POLYMERASE-III [J].

DAS, G ;

HENNING, D ;

WRIGHT, D ;

REDDY, R .

EMBO JOURNAL, 1988, 7 (02) :503-512

[6] Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease P [J].

Eder, PS ;

Kekuda, R ;

Stolc, V ;

Altman, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (04) :1101-1106

[7] NUCLEOTIDE-SEQUENCES OF INFLUENZA-VIRUS SEGMENTS 1 AND 3 REVEAL MOSAIC STRUCTURE OF A SMALL VIRAL-RNA SEGMENT [J].

FIELDS, S ;

WINTER, G .

CELL, 1982, 28 (02) :303-313

[8] EXTERNAL GUIDE SEQUENCES FOR AN RNA ENZYME [J].

FORSTER, AC ;

ALTMAN, S .

SCIENCE, 1990, 249 (4970) :783-786

[9] STRUCTURE IN SOLUTION OF M1 RNA, THE CATALYTIC SUBUNIT OF RIBONUCLEASE-P FROM ESCHERICHIA-COLI [J].

GUERRIERTAKADA, C ;

ALTMAN, S .

BIOCHEMISTRY, 1984, 23 (26) :6327-6334

[10] ARTIFICIAL REGULATION OF GENE-EXPRESSION IN ESCHERICHIA-COLI BY RNASE-P [J].

GUERRIERTAKADA, C ;

LI, Y ;

ALTMAN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :11115-11119

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