Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers

被引:3
作者
Chansiri, K
Kawazu, S
Kamio, T
Fujisaki, K
Panchadcharam, C
Watanapokasin, Y
Uthaisang, W
Tananyutthawongese, C
Sarataphan, N
机构
[1] Srinakharinwirot Univ, Fac Med, Dept Biochem, Bangkok 10110, Thailand
[2] Natl Inst Anim Hlth, Lab Protozoan Dis, Tsukuba, Ibaraki 305, Japan
[3] Obihiro Univ Agr & Vet Med, Obihiro, Hokkaido 080, Japan
[4] Vet Res Inst, Ipoh 31400, Perak, Malaysia
[5] Natl Inst Anim Hlth, Parasitol Sect, Dept Livestock Dev, Bangkok 10900, Thailand
关键词
benign Theileria; randomly amplified polymorphism (RAPD); polymerase chain reaction (PCR); DNA fingerprint; arbitrary primers;
D O I
10.1016/S0304-4017(98)00155-1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:143 / 149
页数:7
相关论文
共 19 条
[1]   GENETIC-VARIABILITY AND EVOLUTION OF THE SCHISTOSOMA GENOME ANALYZED BY USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS [J].
BARRAL, V ;
THIS, P ;
IMBERTESTABLET, D ;
COMBES, C ;
DELSENY, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 59 (02) :211-221
[2]   THEILERIA-PARVA - DETECTION OF GENOMIC POLYMORPHISMS BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF DNA USING ARBITRARY PRIMERS [J].
BISHOP, R ;
SOHANPAL, B ;
MORZARIA, S .
EXPERIMENTAL PARASITOLOGY, 1993, 77 (01) :53-61
[3]  
CALLOW LL, 1984, THEILERIOSIS IN ANIM, P168
[4]   USE OF RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS TO COMPARE BABESIA-BOVIS AND B-BIGEMINA ISOLATES [J].
CARSON, CA ;
BRANDT, HM ;
JENSEN, JB ;
BAILEY, CW ;
ALLEN, GK .
PARASITOLOGY RESEARCH, 1994, 80 (04) :312-315
[5]   THE PRESENCE OF THEILERIA-SERGENTI IN HAEMAPHYSALIS-LONGICORNIS OVERWINTERING IN PASTURE IN JAPAN [J].
FUJISAKI, K ;
ITO, Y ;
KAMIO, T ;
KITAOKA, S .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1985, 79 (05) :519-524
[6]  
Fujisaki K., 1991, P233
[7]   GENETIC COMPARISON OF NEOSPORA-CANINUM WITH TOXOPLASMA AND SARCOCYSTIS BY RANDOM AMPLIFIED POLYMORPHIC - DNA-POLYMERASE CHAIN-REACTION [J].
GUO, ZG ;
JOHNSON, AM .
PARASITOLOGY RESEARCH, 1995, 81 (05) :365-370
[8]   ANALYSIS OF THE GENES ENCODING IMMUNODOMINANT PIROPLASM SURFACE-PROTEINS OF THEILERIA-SERGENTI AND THEILERIA-BUFFELI BY NUCLEOTIDE SEQUENCING AND POLYMERASE CHAIN-REACTION [J].
KAWAZU, S ;
SUGIMOTO, C ;
KAMIO, T ;
FUJISAKI, K .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 56 (01) :169-176
[9]   ANTIGENIC DIFFERENCES BETWEEN JAPANESE THEILERIA-SERGENTI AND OTHER BENIGN THEILERIA SPECIES OF CATTLE FROM AUSTRALIA (T-BUFFELI) AND BRITAIN (T-ORIENTALIS) [J].
KAWAZU, S ;
SUGIMOTO, C ;
KAMIO, T ;
FUJISAKI, K .
PARASITOLOGY RESEARCH, 1992, 78 (02) :130-135
[10]  
Sambrook J., 2002, MOL CLONING LAB MANU