RNA interference directed to CDK2 inhibits HIV-1 transcription

被引：32

作者：

Ammosova, T

Berro, R

Kashanchi, F

Nekhai, S

机构：

[1] Howard Univ, Coll Med, Ctr Sickle Cell Dis, Washington, DC 20059 USA

[2] Howard Univ, Coll Med, Dept Biochem & Mol Biol, Washington, DC 20059 USA

[3] George Washington Univ, Med Ctr, Dept Biochem & Mol Biol, Washington, DC 20037 USA

来源：

VIROLOGY | 2005年 / 341卷 / 02期

关键词：

HIV-1; CDK2; transcription; RNA interference; Tat;

D O I：

10.1016/j.virol.2005.06.041

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously reported that cell cycle-dependent kinase 2 (CDK2) is required for human immunodeficiency virus-1 (HIV-1) Tat-dependent transcription in vitro. In the present study, CDK2-specific RNA interference in cultured HEK293T cells inhibited CDK2 expression and Tat-induced HIV-1 transcription from non-integrated HIV-1 promoter but not basal HIV-1 transcription or transcription from CMV or beta-actin promoters. Also, CDK2-specific RNA interference inhibited Tat-induced transcription from the integrated HIV-1 promoter in HeLa-CD4-LTR-beta-gal cells and potently blocked TNF alpha-induced HIV-1 viral replication in OM 10.1 cells. CDK2-specific RNA interference did not have an effect on cell cycle progression, but it augmented TNF alpha-induced apoptosis of OM 10.1 cells. Our results indicate that CDK2 participates in Tat-mediated HIV-1 transcription and may serve as a potential therapeutic target. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：171 / 178

页数：8

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