In vitro and in vivo analysis of co-electrospun scaffolds made of medical grade poly(ε-caprolactone) and porcine collagen

被引：24

作者：

Chen, Z. C. C. ^{[2
]}

Ekaputra, A. K. ^{[3
]}

Gauthaman, K. ^{[2
]}

Adaikan, P. G. ^{[2
]}

Yu, H. ^{[4
,5
]}

Hutmacher, D. W. ^{[1
]}

机构：

[1] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Kelvin Grove, Qld 4059, Australia

[2] Natl Univ Singapore, Dept Obstet & Gynecol, Singapore 117548, Singapore

[3] Natl Univ Singapore, Grad Program Bioengn, Fac Med, Singapore 117548, Singapore

[4] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117548, Singapore

[5] ASTAR, Inst Bioengn & Nanotechnol, Singapore, Singapore

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2008年 / 19卷 / 05期

关键词：

PCL; collagen; smooth muscle cells; corpus cavernosa; nanofiber; electrospinning; scaffolds;

D O I：

10.1163/156856208784089580

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this study, a nanofiber mesh made by co-electrospinning medical grade poly(epsilon-caprolactone and collagen (mPCL/Col) was fabricated and studied. Its mechanical properties and characteristics were analyzed and compared to mPCL meshes. mPCL/Col meshes showed a reduction in strength but an increase in ductility when compared to PCL meshes. In vitro assays revealed that mPCL/Col supported the attachment and proliferation of smooth muscle cells on both sides of the mesh. In vivo studies in the corpus cavernosa of rabbits revealed that the mPCL/Col scaffold used in conjunction with autologous smooth muscle cells resulted in better integration with host tissue when compared to cell free scaffolds. On a cellular level preseeded scaffolds showed a minimized foreign body reaction.

引用

页码：693 / 707

页数：15

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