OMP decarboxylase - An enigma persists

被引：22

作者：

Callahan, Brian P. ^{[1
]}

Miller, Brian G.

机构：

[1] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA

[2] NYS DOH, Wadsworth Ctr, Div Infect Dis, Albany, NY 12202 USA

来源：

BIOORGANIC CHEMISTRY | 2007年 / 35卷 / 06期

关键词：

decarboxylase; decarboxylation; mechanism; carbanion; carbene; ylide; electrostatic stress; transition state; orotidine; OMP;

D O I：

10.1016/j.bioorg.2007.07.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In 1995, Radzicka and Wolfenden reported that the rate enhancement produced by orotidine 5'-phosphate decarboxylase (ODCase) approaches 1017, making this enzyme the most effective pure protein catalyst known in Nature [A. Radzicka, R. Wolfenden, Science 267 (1995) 90-93]. Over the last 12 years, there have been many hypotheses Put forward to explain that impressive effect. In this perspective, we provide a summary of the reaction pathways under consideration for ODCase, highlight the supporting and refuting data, and suggest experiments designed to further test each of the candidate pathways. Published by Elsevier Inc.

引用

页码：465 / 469

页数：5

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