Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes

被引：4

作者：

Gu, Yingying

Li, Qingge ^{[1
]}

机构：

[1] Xiamen Univ, Mol Diagnost Lab, Dept Biomed Sci, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China

[2] Xiamen Univ, State Key Lab Minist Educ Cell Biol & Tumor Cell, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China

来源：

CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 16-17期

关键词：

aldehyde dehydrogenase-2; genotyping; real-time PCR; displacing probes;

D O I：

10.1016/j.clinbiochem.2007.07.009

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 [基础医学];

摘要：

Objectives: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping. Design and methods: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes. Results: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis. Conclusions: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2. (C) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：1325 / 1327

页数：3

共 10 条

[1]

Real-time PCR genotyping using displacing probes [J].