Diagnosis of human ehrlichiosis by PCR assay of acute-phase serum

被引：28

作者：

Comer, JA ^{[1
]}

Nicholson, WL ^{[1
]}

Sumner, JW ^{[1
]}

Olson, JG ^{[1
]}

Childs, JE ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Viral & Rickettsial Dis, Viral & Rickettsial Zoonoses Branch, US Dept HHS,Natl Ctr Infect Dis, Atlanta, GA 30333 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 01期

关键词：

D O I：

10.1128/JCM.37.1.31-34.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR assay of 43 acute-phase serum samples was evaluated as a method for early detection of human granulocytic ehrlichiosis (HGE) and determination of etiology when serologic testing is inconclusive. Sequence-confirmed products of the HGF agent were amplified from three individuals residing or having exposure history in Minnesota or Wisconsin, and similarly confirmed products from Ehrlichia chaffeensis were amplified from three individuals from Florida or Maryland. Etiology, as determined by PCR and serology, was the same whenever there was a fourfold difference between the maximum titers of antibodies to both antigens, indicating that presumptive determination of etiology may be based on fourfold differences in titers. PCR testing determined that E, chaffeensis was the etiologic agent for one individual who had similar titers of antibodies to both agents. PCR assay of acute-phase serum in the absence of whole blood specimens may be a useful method for early detection of human ehrlichiosis and determination of etiology when serologic testing is inconclusive.

引用

页码：31 / 34

页数：4

共 17 条

[1] DETECTION OF THE ETIOLOGIC AGENT OF HUMAN EHRLICHIOSIS BY POLYMERASE CHAIN-REACTION [J].