An unusual gene arrangement for the putative chromosome replication origin and circadian expression of dnaN in Synechococcus sp strain PCC 7942

被引:14
作者
Liu, Y
Tsinoremas, NF
机构
[1] TEXAS A&M UNIV,DEPT BIOL,COLLEGE STN,TX 77843
[2] VANDERBILT UNIV,DEPT BIOL,NASHVILLE,TN 37235
基金
美国国家科学基金会;
关键词
oriC; DnaA boxes; circadian rhythm; cyanobacteria; luciferase; luxAB;
D O I
10.1016/0378-1119(96)00160-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In eubacteria, the clustering of DnaA boxes around the dnaN (beta subunit of DNA polymerase III) and dnaA genes usually defines the chromosome replication origin (oriC). In this study, the dnaN locus from the cyanobacterium Synechococcus sp. strain PCC 7942 was sequenced. The gene order in this region is cbbZp-dnaN-orf288-purL-purF which contrasts with other eubacteria. A cluster of eleven DnaA boxes (consensus sequence: TTTTCCACA) was found in the intergenic region between dnaN and cbbZp. We also found a 41-bp sequence within this region that is 80% identical to the proposed oriC of Streptomyces coelicolor. Therefore, we propose that this intergenic region may serve as an oriC in Synechococcus. Using bacterial luciferase as a reporter, we also showed that dnaN is rhythmically expressed, suggesting that DNA replication could be under circadian control in this organism.
引用
收藏
页码:105 / 109
页数:5
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