Differential immunohistochemical visualization of the primary and secondary intracellular symbiotic bacteria of aphids

被引：26

作者：

Fukatsu, T ^{[1
]}

Ishikawa, H

机构：

[1] Agcy Ind Sci & Technol, Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 3058566, Japan

[2] Univ Tokyo, Dept Biol Sci, Grad Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

APPLIED ENTOMOLOGY AND ZOOLOGY | 1998年 / 33卷 / 02期

关键词：

aphid; intracellular symbiotic bacteria; primary and secondary symbionts; GroEL and GroES; immunohistochemistry;

D O I：

10.1303/aez.33.321

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

In order to analyze the complex endosymbiotic system of aphids, immunohistochemical double staining was conducted using anti-GroEL and anti-GroES polyclonal antibodies. By this method, primary and secondary intracellular symbiotic bacteria were vividly differentiated by color on the same tissue sections of disymbiotic species from diverse aphid taxa. The primary symbionts were detected with anti-GroEL antiserum in pink while the secondary symbionts were stained with anti-GroES antiserum in dark brown. On account of its wide applicability, and high selectivity and resolution, this method will be a useful tool in investigating the nature, origin and dynamic evolution of the endosymbiotic system in aphids.

引用

页码：321 / 326

页数：6

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