Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.)

被引：144

作者：

Jones, ES ^{[1
]}

Dupal, MP ^{[1
]}

Kölliker, R ^{[1
]}

Drayton, MC ^{[1
]}

Forster, JW ^{[1
]}

机构：

[1] La Trobe Univ, Agr Victoria, Ctr Plant Biotechnol, Bundoora, Vic 3083, Australia

来源：

THEORETICAL AND APPLIED GENETICS | 2001年 / 102卷 / 2-3期

关键词：

simple sequence repeats; SSR-enriched libraries; molecular marker; Lolium perenne; cross-species amplification;

D O I：

10.1007/s001220051661

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Enrichment methods were optimised in order to isolate large numbers of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.), with the aim of developing a comprehensive set of loci for trait mapping and cultivar identification. Two libraries were constructed showing greater than 50% enrichment for a variety of SSR-motif types. Sequence characterisation of 1853 clones identified 859 SSR-containing clones, of which 718 were unique. Truncation of flanking sequences limited potential primer design to 366 clones. One-hundred selected SSR primer pairs were evaluated for amplification and genetic polymorphism across a panel of diverse genotypes. The efficiency of amplification was 81%. A relatively high level of SSR polymorphism was detected (67%), with a range of 2-7 alleles per locus. Mendelian segregation of alleles detected by selected SSR-locus primer pairs was demonstrated in the F-1 progeny of a pair cross. Cross-species amplification was detected in a number of related pasture and turfgrass species, with high levels of transfer to other Lolium species and members of the related genus Festuca. The identity of putative SSR ortholoci in these related species was confirmed by DNA sequence analysis. These loci constitute a valuable resource of ideal markers for the molecular breeding of ryegrasses and fescues.

引用

页码：405 / 415

页数：11

共 60 条

[1]

AKKAYA MS, 1992, GENETICS, V132, P1131

[2] A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers [J].

Bert, PF ;

Charmet, G ;

Sourdille, P ;

Hayward, MD ;

Balfourier, F .

THEORETICAL AND APPLIED GENETICS, 1999, 99 (3-4) :445-452

[3] Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.) [J].

Blair, MW ;

Panaud, O ;

McCouch, SR .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (05) :780-792

[4]

Borrill M., 1976, Evolution of crop plants., P137

[5] Phylogenetic analysis in the Festuca-Lolium complex using molecular markers and ITS rDNA [J].

Charmet, G ;

Ravel, C ;

Balfourier, F .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (08) :1038-1046

[6] Maize simple repetitive DNA sequences: Abundance and allele variation [J].

Chin, ECL ;

Senior, ML ;

Shu, H ;

Smith, JSC .

GENOME, 1996, 39 (05) :866-873

[7] ABUNDANCE AND DNA-SEQUENCE OF 2-BASE REPEAT REGIONS IN TROPICAL TREE GENOMES [J].

CONDIT, R ;

HUBBELL, SP .

GENOME, 1991, 34 (01) :66-71

[8] Optimisation of a microsatellite enrichment technique in Saccharum spp. [J].

Cordeiro, GM ;

Maguire, TL ;

Edwards, KJ ;

Henry, RJ .

PLANT MOLECULAR BIOLOGY REPORTER, 1999, 17 (03) :225-229

[9]

CORDEIRO GM, 1999, 11 AUST PLANT BREED, V2, P208

[10] SELF-INCOMPATIBILITY IN RYEGRASS .1. GENETIC-CONTROL IN DIPLOID LOLIUM-PERENNE L [J].

CORNISH, MA ;

HAYWARD, MD ;

LAWRENCE, MJ .

HEREDITY, 1979, 43 (AUG) :95-&

← 1 2 3 4 5 6 →