Concentration and purification of orotic acid directly from whey with an expanded bed adsorption system

被引：7

作者：

Baumeister, A ^{[1
]}

Vogelmann, S ^{[1
]}

Fischer, L ^{[1
]}

机构：

[1] Univ Hohenheim, Lehrstuhl Biotechnol, Inst Lebensmitteltechnol, D-70599 Stuttgart, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1006卷 / 1-2期

关键词：

whey; expanded bed chromatography; orotic acid; vitamins;

D O I：

10.1016/S0021-9673(03)00945-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of an expanded bed process for the concentration and purification of orotic acid directly from whey is described. Different commercially available adsorbents were tested in series of pilot batch adsorption experiments to determine the most suitable separation system. Best results were achieved using a weak anion-exchange matrix. An elution protocol was established using MgCl2 as eluting agent to recover the adsorbed orotic acid with similar to85% yield and 10-fold concentration. Purified orotic acid was precipitated under acid conditions with a yield of 95%. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：261 / 265

页数：5

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