Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45

被引：158

作者：

Zhang, JH

Liu, XS

Scherer, DC

Van Kaer, L

Wang, XD

Xu, M ^{[1
]}

机构：

[1] Univ Cincinnati, Coll Med, Dept Anat Cell Biol & Neurobiol, Cincinnati, OH 45267 USA

[2] Univ Texas, SW Med Ctr, Dept Biochem, Howard Hughes Med Inst, Dallas, TX 75235 USA

[3] Vanderbilt Univ, Sch Med, Howard Hughes Med Inst, Dept Immunol & Microbiol, Nashville, TN 37232 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 21期

关键词：

D O I：

10.1073/pnas.95.21.12480

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The DNA fragmentation factor 45 (DFF45) is a subunit of a heterodimeric nuclease complex critical for the induction of DNA fragmentation in vitro. To understand the in vivo role of DFF45 in programmed cell death, we generated DFF45 mutant mice. DNA fragmentation activity is completely abolished in cell extracts from DFF45 mutant tissues. In response to apoptotic stimuli, splenocytes, thymocytes, and granulocytes from DFF45 mutant mice are resistant to DNA fragmentation, and splenocytes and thymocytes are also resistant to chromatin condensation. Nevertheless, development of the immune system in the DFF45 mutant mice is normal. These results demonstrate that DFF45 is critical for the induction of DNA fragmentation and chromatin condensation in vivo, but is not required for normal immune system development.

引用

页码：12480 / 12485

页数：6

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[31] Life, death, and the pursuit of apoptosis [J].