Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates

被引：80

作者：

Mohammadi, T

Reesink, HW

Vandenbroucke-Grauls, CMJE

Savelkoul, PHM

机构：

[1] Vrije Univ Amsterdam, Med Ctr, Dept Med Microbiol & Infect Control, NL-1007 MB Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Sanquin Blook Bank NW Reg, NL-1007 MB Amsterdam, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 10期

关键词：

D O I：

10.1128/JCM.41.10.4796-4798.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplitication was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay.

引用

收藏

页码：4796 / 4798

页数：3

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