Folate: methods of analysis

被引:142
作者
Arcot, J [1 ]
Shrestha, A [1 ]
机构
[1] Univ New S Wales, Sch Chem Engn & Ind Chem, Sydney, NSW 2052, Australia
关键词
D O I
10.1016/j.tifs.2005.03.013
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Folates and its derivatives occur as polyglutamates in nature. The multiplicity of forms and the generally low levels in foods makes quantitative analysis of folate a difficult task. The assay of folates from foods generally involves three steps: liberation of folates from the cellular matrix; deconjugation from the polyglutamate to the mono and di-glutamate forms; and the detection of the biological activity or chemical concentration of the resulting folates. The detection methods used are the microbiological assay relying on the turbidimetric bacterial growth of Lactobacillus rhamnosus which is by far the most commonly used method; the HPLC and LC/MS techniques and bio-specific procedures. This review attempts to describe the methods along with the merits and demerits of using each of these methods.
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页码:253 / 266
页数:14
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