Thermal stability studies of the non canonical base pair between 5-methyl-isocytosine and isohypoxanthine

被引：9

作者：

Beaussire, JJ ^{[1
]}

Pochet, S ^{[1
]}

机构：

[1] Inst Pasteur, Unite Chim Organ, F-75724 Paris 15, France

来源：

TETRAHEDRON | 1998年 / 54卷 / 44期

关键词：

D O I：

10.1016/S0040-4020(98)00820-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The hybridization properties of 5-methyl-isocytosine ((5Me)isoC) and isohypoxanthine (isoI) have been investigated by using ultraviolet melting techniques. These two bases were incorporated into heptadecanucleotides using the phosphoramidite chemistry. The base pair (5Me)isoC/isoI was found to be isoenergetic with the A/T base pair. A small destabilization was observed when isoI was located opposite T or G and can be interpreted by a pairing mode between these bases and isoI in its lactam form. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：13547 / 13556

页数：10

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