The ITR binding domain of the mariner Mos-1 transposase

被引：49

作者：

Augé-Gouillou, C ^{[1
]}

Hamelin, MH ^{[1
]}

Demattei, MV ^{[1
]}

Periquet, G ^{[1
]}

Bigot, Y ^{[1
]}

机构：

[1] IRBI Grp Etude Parasites Genet, UPRESA CNRS 6035, Fac Sci, F-37200 Tours, France

来源：

MOLECULAR GENETICS AND GENOMICS | 2001年 / 265卷 / 01期

关键词：

mariner; transposase; DNA-binding properties; transposition;

D O I：

10.1007/s004380000386

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mariner-like elements are widespread eukaryotic transposons, but Mos-1 is the only natural element that is known to be active. Little is known about the biochemistry of mariner transposition. The first step in the process is the binding of the transposase to the 5 ' and 3 ' inverted terminal repeats (ITRs) of the element. Using the 3 ' ITR of the element, we have determined the binding properties of a recombinant Mos-1 transposase produced in bacteria, and we have used deletion derivatives to localize the minimal ITR binding domain between amino acids 1 and 141. Its features and structure indicate that it differs from the ITR binding domain of the transposase encoded by Tc1-related elements.

引用

页码：58 / 65

页数：8

共 23 条

[1]

AUGEGOUILLOU C, 2000, MOL GEN GENET

[2]

BOISSIER F, 1991, J BIOL CHEM, V266, P9822

[3]

CAPY P, 1992, GENETICS, V130, P499

[4] DNA-BINDING ACTIVITIES OF THE CAENORHABDITIS-ELEGANS TC3 TRANSPOSASE [J].

COLLOMS, SD ;

VANLUENEN, HGAM ;

PLASTERK, RHA .

NUCLEIC ACIDS RESEARCH, 1994, 22 (25) :5548-5554

[5]

DESSEN P, 1990, COMPUT APPL BIOSCI, V6, P355

[6] Integrating DNA: Transposases and retroviral integrases [J].

Haren, L ;

Ton-Hoang, B ;

Chandler, M .

ANNUAL REVIEW OF MICROBIOLOGY, 1999, 53 :245-281

[7] Identification of functional domains and evolution of Tc1-like transposable elements [J].

Ivics, Z ;

Izsvak, Z ;

Minter, A ;

Hackett, PB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (10) :5008-5013

[8] MOLECULAR-STRUCTURE OF A SOMATICALLY UNSTABLE TRANSPOSABLE ELEMENT IN DROSOPHILA [J].

JACOBSON, JW ;

MEDHORA, MM ;

HARTL, DL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8684-8688

[9] Hyperactive transposase mutants of the Himar1 mariner transposon [J].

Lampe, DJ ;

Akerley, BJ ;

Rubin, EJ ;

Mekalanos, JJ ;

Robertson, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11428-11433

[10] Purified mariner transposase is sufficient to mediate transposition in vitro [J].

Lampe, DJ ;

Churchill, MEA ;

Robertson, HM .

EMBO JOURNAL, 1996, 15 (19) :5470-5479

← 1 2 3 →