Fast Magnetic Field-Enhanced Linear Colloidal Agglutination Immunoassay

被引：20

作者：

Daynes, Aurelien ^{[1
,2
]}

Temurok, Nevzat ^{[1
]}

Gineys, Jean-Philippe ^{[1
]}

Cauet, Gilles ^{[1
]}

Nerin, Philippe ^{[1
]}

Baudry, Jean ^{[2
]}

Bibettet, Jerome ^{[2
]}

机构：

[1] HORIBA ABX SAS, F-34184 Montpellier 4, France

[2] Lab Colloides & Mat Divises ESPCI, F-75231 Paris, France

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 15期

关键词：

FLOW IMMUNOASSAY; PROTEIN;

D O I：

10.1021/acs.analchem.5b00279

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present the principle of a fast magnetic field enhanced colloidal agglutination assay, which is based on the acceleration of the recognition rate between ligands and receptors induced by magnetic forces.(1) By applying a homogeneous magnetic field of 20 mT for only 7 s, we detect CRP (C-reactive protein) in human serum at a concentration as low as 1 pM for a total cycle time of about 1 min in a prototype analyzer. Such a short measurement time does not impair the performances of the assay when compared to longer experiments. The concentration range dynamic is shown to cover 3 orders of magnitude. An analytical model of agglutination is also successfully fitting our data obtained with a short magnetic pulse.

引用

页码：7583 / 7587

页数：5

共 25 条

[1] Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces
Baudry, J.
Rouzeau, C.
Goubault, C.
Robic, C.
Cohen-Tannoudji, L.
Koenig, A.
Bertrand, E.
Bibette, J.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (44) : 16076 - 16078
[2] Mathematical functions for the analysis of luminescence decays with underlying distributions 1. Kohlrausch decay function (stretched exponential)
Berberan-Santos, MN
Bodunov, EN
Valeur, B
[J]. CHEMICAL PHYSICS, 2005, 315 (1-2) : 171 - 182
[3] Blood pressure, C-reactive protein, and risk of future cardiovascular events
Blake, GJ
Rifai, N
Buring, JE
Ridker, PM
[J]. CIRCULATION, 2003, 108 (24) : 2993 - 2999
[4] Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles
Bruls, D. M.
Evers, T. H.
Kahlman, J. A. H.
van Lankvelt, P. J. W.
Ovsyanko, M.
Pelssers, E. G. M.
Schleipen, J. J. H. B.
de Theije, F. K.
Verschuren, C. A.
van der Wijk, T.
van Zon, J. B. A.
Dittmer, W. U.
Immink, A. H. J.
Nieuwenhuis, J. H.
Prins, M. W. J.
[J]. LAB ON A CHIP, 2009, 9 (24) : 3504 - 3510
[5] A Fast and Sensitive Quantitative Lateral Flow Immunoassay for Cry1Ab Based on a Novel Signal Amplification Conjugate
Chen, Chunxiang
Wu, Jian
[J]. SENSORS, 2012, 12 (09): : 11684 - 11696
[6] Measuring two-dimensional receptor-ligand binding kinetics by micropipette
Chesla, SE
Selvaraj, P
Zhu, C
[J]. BIOPHYSICAL JOURNAL, 1998, 75 (03) : 1553 - 1572
[7] The C-reactive protein
Clyne, B
Olshaker, JS
[J]. JOURNAL OF EMERGENCY MEDICINE, 1999, 17 (06) : 1019 - 1025
[8] Measuring the kinetics of biomolecular recognition with magnetic colloids
Cohen-Tannoudji, L.
Bertrand, E.
Baudry, J.
Robic, C.
Goubault, C.
Pellissier, M.
Johner, A.
Thalmann, F.
Lee, N. K.
Marques, C. M.
Bibette, J.
[J]. PHYSICAL REVIEW LETTERS, 2008, 100 (10)
[9] Measuring colloidal forces with the magnetic chaining technique
Dreyfus, R.
Lacoste, D.
Bibette, J.
Baudry, J.
[J]. EUROPEAN PHYSICAL JOURNAL E, 2009, 28 (02) : 113 - 123
[10] Femtomolar detection of prostate-specific antigen: An immunoassay based on surface-enhanced Raman scattering and immunogold labels
Grubisha, DS
Lipert, RJ
Park, HY
Driskell, J
Porter, MD
[J]. ANALYTICAL CHEMISTRY, 2003, 75 (21) : 5936 - 5943

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