Riboswitches: natural SELEXion

被引：8

作者：

Gilbert, SD ^{[1
]}

Batey, RT ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2005年 / 62卷 / 21期

关键词：

RNA; riboswitch; aptamer; X-ray crystallography; structure;

D O I：

10.1007/s00018-005-5345-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Advances in our knowledge of the structure and chemistry of RNA have been harnessed in the process known as SELEX to develop artificial RNA-based molecules that can act as enzymes and ligand binders performing a wide variety of functions. The discovery of riboswitches, natural RNA aptamers involved in genetic regulation, offers a basis of comparison between the artificial selection and the natural selection of structured RNAs for small-molecule recognition. The guanine riboswitch structural determination allows us to draw conclusions regarding the apparent increased complexity of the riboswitch aptamers compared to their in-vitro-selected cousins.

引用

页码：2401 / 2404

页数：4

共 23 条

[1] Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine [J].

Batey, RT ;

Gilbert, SD ;

Montange, RK .

NATURE, 2004, 432 (7015) :411-415

[2] Evolution of aptamers with secondary structures from a new specificity and new an ATP aptamer [J].

Huang, Z ;

Szostak, JW .

RNA, 2003, 9 (12) :1456-1463

[3] HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA [J].

JENISON, RD ;

GILL, SC ;

PARDI, A ;

POLISKY, B .

SCIENCE, 1994, 263 (5152) :1425-1429

[4] Directed evolution of nucleic acid enzymes [J].

Joyce, GF .

ANNUAL REVIEW OF BIOCHEMISTRY, 2004, 73 :791-836

[5] Role of a heterogeneous free state in the formation of a specific RNA-theophylline complex [J].

Jucker, FM ;

Phillips, RM ;

McCallum, SA ;

Pardi, A .

BIOCHEMISTRY, 2003, 42 (09) :2560-2567

[6] The kink-turn: a new RNA secondary structure motif [J].

Klein, DJ ;

Schmeing, TM ;

Moore, PB ;

Steitz, TA .

EMBO JOURNAL, 2001, 20 (15) :4214-4221

[7]

Koizumi M, 1999, NAT STRUCT BIOL, V6, P1062

[8] Current topics in RNA-protein recognition: Control of specificity and biological function through induced fit and conformational capture [J].

Leulliot, N ;

Varani, G .

BIOCHEMISTRY, 2001, 40 (27) :7947-7956

[9] Adenine riboswitches and gene activation by disruption of a transcription terminator [J].

Mandal, M ;

Breaker, RR .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (01) :29-35

[10] Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria [J].

Mandal, M ;

Boese, B ;

Barrick, JE ;

Winkler, WC ;

Breaker, RR .

CELL, 2003, 113 (05) :577-586

← 1 2 3 →