Enhancement of immune response to naked DNA vaccine by immunization with transfected dendritic cells

被引：120

作者：

Manickan, E ^{[1
]}

Kanangat, S ^{[1
]}

Rouse, RJD ^{[1
]}

Yu, ZY ^{[1
]}

Rouse, BT ^{[1
]}

机构：

[1] UNIV TENNESSEE, DEPT MICROBIOL, COLL VET MED, KNOXVILLE, TN 37996 USA

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1997年 / 61卷 / 02期

关键词：

herpes simplex virus; macrophages; anti-herpes; cytokines; lymphocytes; nucleic acid vaccine; plasmid DNA;

D O I：

10.1002/jlb.61.2.125

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunization with plasmid DNA encoding various proteins promises to be a valuable vaccine approach especially if its immunogenicity could be optimized. In this study we show that the intramuscular delivery in dendritic cells (DC) of naked plasmid DNA encoding two proteins of herpes simplex virus (HSV) leads to the induction of significantly enhanced levels of resistance to viral challenge. Whereas DC transfected in vitro with DNA induced enhanced immunity, similarly transfected macrophage (M phi) populations lacked immunogenicity even though plasmid expression occurred in vitro. The enhanced immunity induced by DC-delivered DNA appeared to be associated mainly with an increased Th1 CD4(+) T cell response. Our results add evidence that DC are the essential antigen-presenting cell types involved in immune responses to intramuscularly administered DNA vaccines.

引用

页码：125 / 132

页数：8

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