Direct NMR detection of the "invisible" alkali metal cations tightly bound to G-quadruplex structures

被引：45

作者：

Wong, A ^{[1
]}

Ida, R ^{[1
]}

Wu, G ^{[1
]}

机构：

[1] Queens Univ, Dept Chem, Kingston, ON K7L 3N6, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 337卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

alkali metal; cation binding; G-quartet; NMR; telomeric DNA;

D O I：

10.1016/j.bbrc.2005.08.275

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the first direct solution NMR detection of the alkali metal cations (Na-23(+), K-39(+), and Rb-87(+)) residing inside G-quadruplex channel structures formed by guanosine 5 '-monophosphate and a DNA oligomer, d(TG(4)T). In solution, these channel alkali metal cations are tightly bound to the G-quadruplex structure and have been considered to be "invisible" to NMR spectroscopy for many years. Our finding that it is possible to directly observe these alkali metal cations by NMR spectroscopy provides a new tool for studying cation binding affinity and dynamics in G-quadruplex DNA. (c) 2005 Elsevier Inc. All rights reserved.

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页码：363 / 366

页数：4

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