Novel method of detecting mismatch on DNA by using photo-induced electron transfer through DNA

被引：12

作者：

Ihara, T ^{[1
]}

Takata, J ^{[1
]}

Takagi, M ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Chem Syst & Engn, Higashi Ku, Fukuoka 8128581, Japan

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 365卷 / 1-3期

关键词：

double stranded DNA; mismatch; mispairing; electron transfer; fluorescence quenching; porphyrin; dimethylphananthrolinium; Stern-Volmer constant;

D O I：

10.1016/S0003-2670(97)00621-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Novel methodology for detecting a defect on double stranded DNA was presented, the principle being based on the dependence of the efficiency of photo-induced electron transfer through double stranded DNA on the structural disorders in the base stacking. The efficiency of photo-induced electron transfer between 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphine tetrachloride and N,N'-dimethyl-4,7-phenanthrolinium dichloride on double stranded DNA was changed by several base-mispairing on the double stranded DNA. This phenomenon will be useful for the development of the convenient method of detecting mismatch on DNA duplex in homogeneous solution. (C) 1998 Elsevier Science B.V.

引用

页码：49 / 54

页数：6

共 26 条

[1]

[Anonymous], 1989, DNA PROBES

[2] DYNAMICS OF ELECTRON-TRANSFER BETWEEN INTERCALATED POLYCYCLIC MOLECULES - EFFECT OF INTERSPERSED BASES [J].

BRUN, AM ;

HARRIMAN, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3656-3660

[3] ENERGY-TRANSFER AND ELECTRON-TRANSFER PROCESSES INVOLVING PALLADIUM PORPHYRINS BOUND TO DNA [J].

BRUN, AM ;

HARRIMAN, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (23) :10383-10393

[4] OLIGONUCLEOTIDE INTERACTIONS .3. CIRCULAR DICHROISM STUDIES OF CONFORMATION OF DEOXYOLIGONUCLEOTIDES [J].

CANTOR, CR ;

WARSHAW, MM ;

SHAPIRO, H .

BIOPOLYMERS, 1970, 9 (09) :1059-&

[5] PHOTOINDUCED ELECTRON-TRANSFER IN DNA MATRIX FROM INTERCALATED ETHIDIUM TO CONDENSED METHYLVIOLOGEN [J].

FROMHERZ, P ;

RIEGER, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (17) :5361-5362

[6]

HUNTER WN, 1987, J BIOL CHEM, V262, P9962

[7] STRUCTURE OF AN ADENINE.CYTOSINE BASE PAIR IN DNA AND ITS IMPLICATIONS FOR MISMATCH REPAIR [J].

HUNTER, WN ;

BROWN, T ;

ANAND, NN ;

KENNARD, O .

NATURE, 1986, 320 (6062) :552-555

[8] REFINED CRYSTAL-STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH G.T MISMATCHED BASE-PAIRS [J].

HUNTER, WN ;

KNEALE, G ;

BROWN, T ;

RABINOVICH, D ;

KENNARD, O .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 190 (04) :605-618

[9] Ferrocene-oligonucleotide conjugates for electrochemical probing of DNA [J].

Ihara, T ;

Maruo, Y ;

Takenaka, S ;

Takagi, M .

NUCLEIC ACIDS RESEARCH, 1996, 24 (21) :4273-4280

[10]

IHARA T, 1997, J CHEM SOC CHEM COMM, P1609

← 1 2 3 →