Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

被引：7

作者：

Antich-Rossello, Miquel ^{[1
,2
]}

Forteza-Genestra, Maria Antonia ^{[1
,2
]}

Calvo, Javier ^{[1
,2
,3
]}

Gaya, Antoni ^{[1
,2
,3
]}

Monjo, Marta ^{[1
,2
,4
]}

Ramis, Joana Maria ^{[1
,2
,4
]}

机构：

[1] Univ Balearic Isl, Cell Therapy & Tissue Engn Grp, Res Inst Hlth Sci IUNICS, Palma De Mallorca, Spain

[2] Hlth Res Inst Balearic Isl IdISBa, Palma De Mallorca, Spain

[3] Fundacio Banc Sang & Teixits Illes Balears FBSTIB, Palma De Mallorca, Spain

[4] Univ Balearic Isl, Dept Fundamental Biol & Hlth Sci, Palma De Mallorca, Spain

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2021年 / 174期

关键词：

D O I：

10.3791/62781

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Extracellular Vesicles (EVs) are biological nanovesicles that play a key role in cell communication. Their content includes active biomolecules such as proteins and nucleic acids, which present great potential in regenerative medicine. More recently, EVs derived from Platelet Lysate (PL) have shown an osteogenic capability comparable to PL. Besides, biomaterials are frequently used in orthopedics or dental restoration. Here, we provide a method to functionalize Ti surfaces with PL-derived EVs in order to improve their osteogenic properties. EVs are isolated from PL by size exclusion chromatography, and afterward Ti surfaces are functionalized with PL-EVs by drop casting. Functionalization is proven by EVs release and its biocompatibility by the lactate dehydrogenase (LDH) release assay.

引用

页数：11

共 16 条

[1]

Platelet-derived extracellular vesicles promote osteoinduction of mesenchymal stromal cells [J].