Volume cytometry: Microfluidic sensor for high-throughput screening in real time

被引:41
作者
Ateya, DA
Sachs, F
Gottlieb, PA
Besch, S
Hua, SZ [1 ]
机构
[1] SUNY Buffalo, Dept Mech & Aerosp Engn, BioMEMS & Biomat Lab, Buffalo, NY 14260 USA
[2] SUNY Buffalo, Dept Physiol & Biophys, Ctr Single Mol Biophys, Buffalo, NY 14214 USA
关键词
D O I
10.1021/ac048799a
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Regulation of cell volume was one of the earliest evolutionary demands for life and remains a universal measure of cell metabolism. Since conventional methods to measure cell volume, such as microscopy, are complex and time-consuming, cell volume has not been used as the basis for cell-based screening. We have developed a microfabricated chip that can measure the volume of small numbers of cells in real time with unprecedented resolution. The method is applicable to adherent or suspended populations of cells and membrane-bound organelles. Our prototype device can detect volume changes in a monolayer of tissue-cultured astrocytes responding to anisotonic stimuli of <1m0sm. We determined the sensitivity to antibiotics of different E. coli strains in <10 min at 24 degreesC. This time can be reduced at higher temperatures enabling on-site clinical testing of infectious agents. Using the chip to screen natural products, we found a peptide in spider venom that inhibits eukaryotic volume regulation at similar to100pM. The prototype chip made in silicon is inexpensive, reusable, and runs on low-voltage electrical power. The technology can be readily transferred to large arrays in plastic.
引用
收藏
页码:1290 / 1294
页数:5
相关论文
共 28 条
[1]   Acute ethanol exposure induces [Ca2+]i transients, cell swelling and transformation of actin cytoskeleton in astroglial primary cultures [J].
Allansson, L ;
Khatibi, S ;
Olsson, T ;
Hansson, E .
JOURNAL OF NEUROCHEMISTRY, 2001, 76 (02) :472-479
[2]   Methylmercury-induced inhibition of regulatory volume decrease in astrocytes: characterization of osmoregulator efflux and its reversal by amiloride [J].
Aschner, M ;
Vitarella, D ;
Allen, JW ;
Conklin, DR ;
Cowan, KS .
BRAIN RESEARCH, 1998, 811 (1-2) :133-142
[3]   Necrotic volume increase and the early physiology of necrosis [J].
Barros, LF ;
Hermosilla, T ;
Castro, J .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 2001, 130 (03) :401-409
[4]   The role of apoptotic volume decrease and ionic homeostasis in the activation and repression of apoptosis [J].
Bortner, CD ;
Cidlowski, JA .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2004, 448 (03) :313-318
[5]   Opposite modulation of NMDA receptors by lysophospholipids and arachidonic acid: common features with mechanosensitivity [J].
Casado, M ;
Ascher, P .
JOURNAL OF PHYSIOLOGY-LONDON, 1998, 513 (02) :317-330
[6]  
Cole KS., 1972, Membranes, Ions and Impulses: A Chapter of Classical Biophysics
[7]  
CORNET M, 1993, J MEMBRANE BIOL, V133, P161
[8]  
Coulter WH., 1953, Means for counting particles suspended in a fluid, Patent No. [2656508, 2656508A]
[9]   VOLUME CHANGES IN SINGLE N1E-115 NEUROBLASTOMA-CELLS MEASURED WITH A FLUORESCENT-PROBE [J].
CROWE, WE ;
ALTAMIRANO, J ;
HUERTO, L ;
ALVAREZLEEFMANS, FJ .
NEUROSCIENCE, 1995, 69 (01) :283-296
[10]   COUNTING AND SIZING OF SUBMICRON PARTICLES BY RESISTIVE PULSE TECHNIQUE [J].
DEBLOIS, RW ;
BEAN, CP .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1970, 41 (07) :909-&