Structural and functional studies of gap junction channels

被引：66

作者：

Nakagawa, So ^{[1
]}

Maeda, Shoji ^{[1
]}

Tsukihara, Tomitake ^{[1
,2
]}

机构：

[1] Osaka Univ, Inst Prot Res, OLABB, Suita, Osaka 5650874, Japan

[2] Univ Hyogo, Dept Life Sci, Akoh, Hyogo 6781297, Japan

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2010年 / 20卷 / 04期

关键词：

DOMINANT CONGENITAL CATARACT; EXTRACELLULAR LOOP DOMAIN; OCULODENTODIGITAL DYSPLASIA; GATING POLARITY; N-TERMINUS; ERYTHROKERATODERMIA VARIABILIS; 3-DIMENSIONAL STRUCTURE; SENSORINEURAL DEAFNESS; CONNEXIN HEMICHANNELS; PULVERULENT CATARACT;

D O I：

10.1016/j.sbi.2010.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

X-ray analysis of the human connexin26 gap junction channel has provided structural details of its open state. The gap junction channel is formed by paired hemichannels on two adjacent cells; each hemichannel consists of six protomers, and exhibits a six-fold symmetry. The protomer folds in a typical four-helix bundle. The amino-terminal region folds in a short helix and is inserted into the lumen to form a funnel structure. The structure of the amino-terminal region could explain the channel's gating mechanism. Extensive interactions between two hemichannels allow the gap junction channel to tightly connect two adjacent cells. The gap junction, which consists of hundreds of gap junction channels, could both serve as an intracellular channel and contribute to cellular adhesion.

引用

页码：423 / 430

页数：8

共 47 条

[1]

ELECTRON MICROSCOPIC OBSERVATIONS ON NEGATIVELY STAINED PLASMA MEMBRANES ISOLATED FROM RAT LIVER [J].

BENEDETT.EL ;

EMMELOT, P .

JOURNAL OF CELL BIOLOGY, 1965, 26 (01) :299-&

[2]

Functional analysis of a dominant mutation of human connexin26 associated with nonsyndromic deafness [J].

Bruzzone, R ;

Gomès, D ;

Denoyelle, F ;

Duval, N ;

Perea, J ;

Veronesi, V ;

Weil, D ;

Petit, C ;

Gabellec, MM ;

D'Andrea, P ;

White, TW .

CELL COMMUNICATION AND ADHESION, 2001, 8 (4-6) :425-+

[3]

A novel mutation in the Connexin 46 gene causes autosomal dominant congenital cataract with incomplete penetrance [J].

Burdon, KP ;

Wirth, MG ;

Mackey, DA ;

Russell-Eggitt, IM ;

Craig, JE ;

Elder, JE ;

Dickinson, JL ;

Sale, MM .

JOURNAL OF MEDICAL GENETICS, 2004, 41 (08) :e106

[4]

MUTATIONAL ANALYSIS OF GAP JUNCTION FORMATION [J].

DAHL, G ;

WERNER, R ;

LEVINE, E ;

RABADANDIEHL, C .

BIOPHYSICAL JOURNAL, 1992, 62 (01) :172-182

[5]

CELL CELL CHANNEL FORMATION INVOLVES DISULFIDE EXCHANGE [J].

DAHL, G ;

LEVINE, E ;

RABADANDIEHL, C ;

WERNER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 197 (01) :141-144

[6]

Devi R, 2005, MOL VIS, V11, P846

[7]

Devi RR, 2006, MOL VIS, V12, P190

[8]

Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels [J].

Dong, Lixian ;

Liu, Xiaoqin ;

Li, Hui ;

Vertel, Barbara A. ;

Ebihara, Lisa .

JOURNAL OF PHYSIOLOGY-LONDON, 2006, 576 (03) :787-799

[9]

Gap junction adhesion is necessary for radial migration in the neocortex [J].

Elias, Laura A. B. ;

Wang, Doris D. ;

Kriegstein, Arnold R. .

NATURE, 2007, 448 (7156) :901-U3

[10]

Connexin30 mutations responsible for hidrotic ectodermal dysplasia cause abnormal hemichannel activity [J].

Essenfelder, GM ;

Bruzzone, R ;

Lamartine, J ;

Charollais, A ;

Blanchet-Bardon, C ;

Barbe, MT ;

Meda, P ;

Waksman, G .

HUMAN MOLECULAR GENETICS, 2004, 13 (16) :1703-1714

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