Steady state and time-resolved fluorescence investigation of the specific binding of two chlorin derivatives with human serum albumin

被引：91

作者：

Patel, Sunita ^{[1
]}

Datta, Anindya ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Bombay 400076, Maharashtra, India

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2007年 / 111卷 / 35期

关键词：

D O I：

10.1021/jp072544u

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The specific binding of two model drugs for photodynamic therapy, namely chlorin P-6 and purpurin 18 in the vicinity of Sudlow's Site I of HSA has been investigated by monitoring the intrinsic fluorescence of single tryptophanyl residue and by competitive binding with warfarin. The distance from the tryptophanyl residue has been ascertained by FRET from Trp to the chlorins and has been found to indicate a binding to Sudlow's Site i. The principal driving force for the interaction is found to be the hydrophobic effect. The main mechanism of protein fluorescence quenching was static. Time-resolved fluorescence results of competitive binding with warfarin are found to confirm that they bind to the warfarin binding site.

引用

页码：10557 / 10562

页数：6

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