Airway remodelling in children with cystic fibrosis

被引:106
作者
Hilliard, Tom N.
Regamey, Nicolas
Shute, Janis K.
Nicholson, Andrew G.
Alton, Eric W. F. W.
Bush, Andrew
Davies, Jane C.
机构
[1] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, Dept Gene Therapy, London, England
[2] Univ Portsmouth, Inst Biomed & Biomol Sci, Portsmouth, Hants, England
[3] Royal Brompton Hosp, Dept Histopathol, London SW3 6LY, England
[4] Royal Brompton Hosp, Dept Paediat Resp Med, London SW3 6LY, England
基金
英国医学研究理事会;
关键词
D O I
10.1136/thx.2006.074641
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: The relationship between airway structural changes and inflammation is unclear in early cystic fibrosis (CF) lung disease. A study was undertaken to determine changes in airway remodelling in children with CF compared with appropriate disease and healthy controls. Methods: Bronchoalveolar lavage and endobronchial biopsy were performed in a cross-sectional study of 43 children with CF (aged 0.3 - 16.8 years), 7 children with primary ciliary dyskinesia (PCD), 26 with chronic respiratory symptoms (CRS) investigated for recurrent infection and/or cough and 7 control children with no lower airway symptoms. Inflammatory cells, cytokines, proteases and matrix constituents were measured in bronchoalveolar lavage fluid (BALF). Reticular basement membrane (RBM) thickness was measured on biopsy specimens using light microscopy. Results: Increased concentrations of elastin, glycosaminoglycans and collagen were found in BALF from children with CF compared with the CRS group and controls, each correlating positively with age, neutrophil count and proteases (elastase activity and matrix metalloproteinase-9 (MMP-9) concentration). There were significant negative correlations between certain of these and pulmonary function (forced expiratory volume in 1 s) in the CF group (elastin: r = -0.45, p<0.05; MMP-9: TIMP-1 ratio: r = -0.47, p< 0.05). Median RBM thickness was greater in the CF group than in the controls (5.9 mu m vs 4.0 mu m, p<0.01) and correlated positively with levels of transforming growth factor-beta(1) (TGF-beta(1); r=0.53, p=0.01), although not with other inflammatory markers or pulmonary function. Conclusions: This study provides evidence for two forms of airway remodelling in children with CF: (1) matrix breakdown, related to inflammation, proteolysis and impaired pulmonary function, and (2) RBM thickening, related to TGF-beta(1) concentration but independent of other markers of inflammation.
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收藏
页码:1074 / 1080
页数:7
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