A new method for reliable and efficient reconstruction of 3-dimensional images from autoradiographs of brain sections

被引：28

作者：

Hess, A

Lohmann, K

Gundelfinger, ED

Scheich, H

机构：

[1] Leibniz Inst Neurobiol, D-39008 Magdeburg, Germany

[2] Otto Von Guericke Univ, Fac Med, D-39118 Magdeburg, Germany

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1998年 / 84卷 / 1-2期

关键词：

digital image processing; three dimensional reconstructions; alignment; 2-fluoro-2-deoxyglucose; auditory cortex; gerbil; autoradiography;

D O I：

10.1016/S0165-0270(98)00102-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Valuable information on metabolism and function of distinct brain regions can be extracted from autoradiographs of 2D brain sections, e.g. after labelling with the non-metabolisable sugar derivate [(14)C]-2-fluoro-deoxyglucose (2-FDG). For a more complete comprehension of the data and for a comparison with information obtained by modern functional imaging techniques it is essential to have a reliable and efficient method for the 3D reconstruction of autoradiographs of serial sections. This paper describes a new method for the alignment of 2D 2-FDG images, that combines two established algorithms, i.e. principal axes alignment followed by consistent matrix transformation. The power and efficiency of this new 2-step method is compared to those of various previously described procedures(1). (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：77 / 86

页数：10

共 17 条

[1] COMPUTER-ASSISTED ALIGNMENT OF STANDARD SERIAL SECTIONS WITHOUT USE OF ARTIFICIAL LANDMARKS - A PRACTICAL APPROACH TO THE UTILIZATION OF INCOMPLETE INFORMATION IN 3-D RECONSTRUCTION OF THE HIPPOCAMPAL REGION [J].