α-D-Glcp-(1⇆1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

被引：42

作者：

Fransen, CTM

Van Laere, KMJ

van Wijk, AAC

Brüll, LP

Dignum, M

Thomas-Oates, JE

Haverkamp, J

Schols, HA

Voragen, AGJ

Kamerling, JP

Vliegenthart, JFG

机构：

[1] Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands

[2] Wageningen Univ Agr, Food Sci Grp, NL-6700 EV Wageningen, Netherlands

[3] Univ Utrecht, Bijvoet Ctr, Dept Mass Spectrometry, NL-3584 CA Utrecht, Netherlands

来源：

CARBOHYDRATE RESEARCH | 1998年 / 314卷 / 1-2期

关键词：

beta-galactosidase; transgalactosylation; functional food; non-reducing galacto-oligosaccharides; oligogalactosylated glucoses; primary structure analysis;

D O I：

10.1016/S0008-6215(98)00285-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)](n)-alpha-D-Glcp-(1 <----> 1)-beta-D-Galp[-(4<--1)-beta-D-Galp](m), with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp-(1 <----> 1)-beta-D-Galp. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：101 / 114

页数：14

共 22 条

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