Sulfonylureas blockade of neural and cardiac HERG channels

The human ether-a-go-go-related gene (herg) encodes a K+ current (I-HERG) which plays a fundamental role in heart excitability and in neurons by contributing to action potential repolarization and to spike-frequency adaptation, respectively. In this paper we show that I-HERG, recorded in neuroblastoma cells and guinea-pig ventricular myocytes, was reversibly inhibited by the K-ATP channel blocker glibenclamide (IC50 = 74 mu M). The voltage and use dependence of glibenclamide blockade mere also evaluated. Another sulfonylurea, glimepiride, had less effective results in blocking I-HERG. The findings of this study are relevant to the interpretation of glibenclamide effects on cellular electrophysiology and suggest that oral antidiabetic therapy with sulfonylureas may contribute to iatrogenic QT prolongation and related arrhythmias. (C) 1998 Federation of European Biochemical Societies.