A double-axis microscope and its three-dimensional image position adjustment based on an optical marker method

被引：3

作者：

Kikuchi, S

Sonobe, K

Shinohara, D

Ohyama, N

Mashiko, S

Hiraoka, Y

机构：

[1] OLYMPUS OPT CO LTD,PROD DEV DEPT,HACHIOJI,TOKYO 192,JAPAN

[2] TOKYO INST TECHNOL,IMAGING SCI & ENGN LAB,MIDORI KU,YOKOHAMA,KANAGAWA 226,JAPAN

[3] KANSAI ADV RES CTR,COMMUN RES LAB,NISHI KU,KOBE 65124,JAPAN

来源：

OPTICS COMMUNICATIONS | 1996年 / 129卷 / 3-4期

关键词：

D O I：

10.1016/0030-4018(96)00150-2

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a double-axis microscope, in which two microscopic imaging systems are orthogonally placed, for reconstructing 3D images of micro specimens. We first show the optical properties of the microscope by analyzing 3D observation images of a small fluorescent sphere. We next propose a position adjustment method for 3D images taken through the microscope using the small sphere as an optical marker.

引用

页码：237 / 244

页数：8

共 11 条

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AGARD DA, 1984, ANNU REV BIOPHYS BIO, V13, P191

[2]

[Anonymous], 1990, CONFOCAL MICROSCOPY

[3]

[Anonymous], 1993, Three-Dimensional Computer Vision: A Geometric Viewpoint

[4] A VERSATILE 2-PI-TILTING DEVICE FOR FLUORESCENCE MICROSCOPES [J].