Identification of a 2,6-dichloroisonicotinic-acid-sensitive protein kinase from tobacco by affinity chromatography on benzothiadiazole-sepharose and NIM-metal chelate adsorbent

被引：6

作者：

Pillonel, C ^{[1
]}

机构：

[1] Novartis Crop Protect Ltd, CH-4002 Basel, Switzerland

来源：

PEST MANAGEMENT SCIENCE | 2001年 / 57卷 / 08期

关键词：

systemic acquired resistance; protein kinase; 2,6-dichloroisonicotinic acid; benzothiadiazoles; salicylic acid; tobacco;

D O I：

10.1002/ps.347

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In the search for the target site of inducers of systemic acquired resistance (SAR), a BTH-binding protein kinase (BBPK) has been identified from tobacco by affinity chromatography on benzothiadiazole-sepharose (CGA 324041-sepharose) and NIM-metal chelate affinity resin. The substrate selectivity of the isolated enzyme (phosphorylation of histone type III-S, I kappaB alpha ,IkB alpha S32A/ S36A and NIM1) suggested a possible BBPK-mediated regulation of NIM1 in tobacco. The measurement of the effect of different SAR-inducers showed an inhibition of BBPK by 2,6-dichloroisonicotinic acid (INA) and, to a lower extent, by benzothiadiazoles and salicylic acid. Comparison between BBPK cell-free inhibition and in vivo PR-1 induction revealed that BBPK could be the target site of INA. (C) 2001 Society of Chemical Industry.

引用

页码：676 / 682

页数：7

共 31 条

[1] Inhibition of IκB kinase activity by sodium salicylate in vitro does not reflect its inhibitory mechanism in intact cells [J].

Alpert, D ;

Vilcek, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (15) :10925-10929

[2]

Bauerle PA, 1996, CELL, V87, P13

[3] The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats [J].

Cao, H ;

Glazebrook, J ;

Clarke, JD ;

Volko, S ;

Dong, XN .

CELL, 1997, 88 (01) :57-63

[4] Protein dephosphorylation mediates salicylic acid-induced expression of PR-1 genes in tobacco [J].

Conrath, U ;

Silva, H ;

Klessig, DF .

PLANT JOURNAL, 1997, 11 (04) :747-757

[5] 2 INDUCERS OF PLANT DEFENSE RESPONSES, 2,6-DICHLOROISONICOTINIC ACID AND SALICYLIC-ACID, INHIBIT CATALASE ACTIVITY IN TOBACCO [J].

CONRATH, U ;

CHEN, ZX ;

RICIGLIANO, JR ;

KLESSIG, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7143-7147

[6]

Cult J. R., 1992, PLANT GENE RES GENES, P209

[7] ARABIDOPSIS SIGNAL-TRANSDUCTION MUTANT DEFECTIVE IN CHEMICALLY AND BIOLOGICALLY INDUCED DISEASE RESISTANCE [J].

DELANEY, TP ;

FRIEDRICH, L ;

RYALS, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) :6602-6606

[8] Identification of a soluble, high-affinity salicylic acid-binding protein in tobacco [J].

Du, H ;

Klessig, DF .

PLANT PHYSIOLOGY, 1997, 113 (04) :1319-1327

[9] INHIBITION OF ASCORBATE PEROXIDASE BY SALICYLIC-ACID AND 2,6-DICHLOROISONICOTINIC ACID, 2 INDUCERS OF PLANT DEFENSE RESPONSES [J].

DURNER, J ;

KLESSIG, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :11312-11316

[10] A benzothiadiazole derivative induces systemic acquired resistance in tobacco [J].

Friedrich, L ;

Lawton, K ;

Ruess, W ;

Masner, P ;

Specker, N ;

Rella, MG ;

Meier, B ;

Dincher, S ;

Staub, T ;

Uknes, S ;

Metraux, JP ;

Kessmann, H ;

Ryals, J .

PLANT JOURNAL, 1996, 10 (01) :61-70

← 1 2 3 4 →